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Anti Rpn7 (S.cerevisiae) polyclonal antibody


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Background

The 26 S proteasome is a protein complex with a molecular mass of -2000 kDa and is highly conserved among eukaryotic organisms. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (1). Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle (1, 2). The RPN7 gene is known to be essential and required for the integrity of the 26 S complex by establishing a correct lid structure (3, 4).

Application

1) Western blotting (-1000 fold dilution)
2) Immunoprecipitation
Not tested for other applications

Detection of Rpn7 (49 kDa) in the crude extract of S. cerevisiae by Western blotting using this antibody.

<Reference>
This product was used in ref.3 and 4.
1. Hershko, A., and Ciechanover, A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67, 425-479 (1998)
2. Finley D, et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E, et al. Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae J Biol Chem. 279:27168-76 (2004)
4. Isono E, et al. The assembly pathway of

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Product Name Cat# Quantity Price

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