Anti RBP2/JARID1A monoclonal antibody


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Background

RBP2 was originally identified as a RB binding protein. It is also known as JARID1A (Jumonji, AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation, depending on the kinds of genes and regulates differentiation by its function as the H3K 4histone demethylase (1,2,3).

Application

1. Western blotting (~1삇/ml)
2. Other applications were not tested.

<Identification of RBP2 in crude cell extracts by western blotting with antibody 9A6 and 18E8.>

A : SDS-PAGE with CBB staining
Samples 1. HeLa control siRNA
Samples 2. HeLa RBP2 siRNA
Samples 3. MCF7
Samples 4. U2OS
Samples 5. NIH3T3
Samples 6. J1 (mouse ES)

B : Western blotting with 9A6

C : Western blotting with 18E8

<Reference>
1. Lopez-Bigas N et al. Genome-wide analysis of the H3K4 demethylase RBP2 reveals a transcriptional program controlling differentiation. Moll Cell 31: 520-30 (2008)
2. Klose RJ et al. The retinoblastoma binding protein BRP2 is an H3K4 demethylase. Cell 128: 889-900 (2007)
3. Christensen J. et al. RBP2 belongs to a family of demethylases, specific tri- and dimethylated lysine 4 on histone 3. Cell 128:1063-76 (2007)

Product List

Product Name Cat# Quantity Price

Anti KDM5A/RBP2/JARID1A, Human (Mouse) Unlabeled DataSheet

BAM-71-175-EX

50 UG see also
# BAM-71175EX
Size 100

Anti KDM5A/RBP2/JARID1A, Human (Mouse) Unlabeled DataSheet

BAM-71-177-EX

50 UG see also
# BAM-71177EX
Size 100

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



“Anti RBP2/JARID1A monoclonal antibody” also belongs to the following categories.