Anti Nup153 monoclonal antibody


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Background

The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.

Application

1) Western blotting (160 kDa single band in Hela cell extract)
2) Immunocytochemistry
3) ELISA
Other applications were not tested
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.

<Western blotting>

Sample is the nuclear membrane fraction of HeLa cells (Left) and NIH3T3 cells (Right).


Western blotting
Sample :
Left : the nuclear membrane fraction of HeLa cells
Right : NIH3T3 cells

Immunofluorescent : HeLa cells

Iimmuno-fluorescence : HeLa cells

<Reference>
1. Ullman,K.S. et al. (1999) “The nucleoporin Nup153 plays a critical role in multiple types of nuclear export.” Molecular Biology of the Cell 10: 649-664.
2. Lim,R.Y. et al. (2007). "Nanomechanical basis of selective gating by the nuclear pore complex." Science 318: 640-643.

Product List

Product Name Cat# Quantity Price

Anti Nup153, Rat (Rat)  DataSheet

BAM-70-315-EX

200 UG ¥34,500
$460

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Product Name Cat# Quantity Price

Anti Lamin B Receptor, Mouse (Rabbit) Unlabeled DataSheet

BAM-70-301-EX

50 UG ¥29,000
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Anti Nup153, Rat (Rat)  DataSheet

BAM-70-315-EX

200 UG ¥34,500
$460

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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