Anti Dnmt1 (1037-1386) polyclonal antibod


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Background

Dnmt1 (DNA (cytosine-5-)-methyltransferase 1) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics). Hypermethylation in promoter regions is asssociated with repression of gene expression. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1, 2). This antibody was raised by Prof. S. Tajima of Osaka Univ. (3, 4)

Application

1) Western blotting (0.2~1 ug /ml)
2) Immunoprecipitation Especially good for denatured protein and useful for ChiP assays
3) Indirect immuno-fluorescence staining


Immunodetection of Dnmt1
1. Murine erythrolekemia cells, MEL11A2
2. Mouse myoblast cells,C2C12
3. C2C12 carrying Dnmt1 overexpressing plasmid
4. C2C12 carrying negative control plasmid
1~4; Western blotting.
5~8; Immunoprecipitation of SDS denatured Dnmt1.

<Reference>
This product was used in Ref.3 and 4
1. Di Croce L, et al. (2002). Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor. Science 295: 1079-82.
2. Rhee I, et al. (2002). DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Nature 416: 552-6.
3. Takagi H, et al (1995) Overexpression of DNA methyltransferase in myoblast cells accelerates myotube formation. Eur. J. Biochem. 282-91
4. Inano K, et al (2000) Maintenance-type DNA methyltransferase is highly expressed in post-mitotic neurons and localized in the cytoplasmic compartment. J. Biochem. 128: 315-21

Product List

Product Name Cat# Quantity Price

Anti Dnmt1 (1037-1386), Mouse (Rabbit) Unlabeled DataSheet

BAM-70-203-EX

50 UG ¥29,000
$387

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