Anti HP1Beta/CBX1 polyclonal antibody


print

Background

Heterochromatin protein 1 (HP1) is a major component of heterochromatin play a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionarily conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family of proteins is encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1β is encoded by the Chromobox homolog 1 (CBX1) gene located on chromosome 17q21.32. HP1βhas been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (1).
The product is prepared by immunizing rabbit with the synthetic peptide CNEDDDKKDDKN including the C-terminal sequence (176-185) of human HP1β(3) and by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi (Ref. 3).

Application

1) Western blotting (1/2000~1/10000)
2) Immunofluoresece staining
3) Chromatin immunoprecipitation (ChIP) (Ref. 3)


Western blotting.
Sample : Extract of MCF7 cellsThe antibody was used at 1000 fold dilution

Immunofluorescent staining
Sample : HP1βin Baby Hamster Kidney cells
Cells were fixed with para-formaldehyde.
The second antibody : Alexa Fluor 594- conjugated goat anti-rabbit IgG

<Reference>
1. Lomberk G et al. Genome Biol. 7, 228 (2006), review
2. Kametaka A et al. Genes Cells 7, 1231 (2002)
3. Wang F et al. Mol. Cell. Biol. 26, 4028 (2006)

Product List

Product Name Cat# Quantity Price

Anti HP1 (β)/CBX1, Human (Rabbit)  DataSheet

BAM-70-223-EX

50 UG ¥21,000
$280

Related Products


Product Name Cat# Quantity Price

Anti Swi6 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-101-EX

50 UL ¥30,000
$400

Anti Swi6 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-102-EX

250 UL N/A

Anti Cut5/Rad4 Saccharomyces cerevisiae  (Rabbit)  DataSheet

BAM-63-107-EX

100 UL ¥35,000
$467

Anti Dis1 Schizosaccharomyces pombe  (Rabbit) Unlabeled

BAM-63-117-EX

200 UL contact us

Anti DIS3 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-123EX

100 UL ¥30,000
$400

Anti H2B Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-125-EX

50 UL ¥30,000
$400

Anti Pad Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-133-EX

100 UL ¥35,000
$467

Anti Ppa2 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-135-EX

100 UL ¥35,000
$467

Anti Psm1 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-137-EX

100 UL ¥35,000
$467

Anti Sds22 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-141-EX

100 UL ¥35,000
$467

Anti Mrc1 Schizosaccharomyces pombe  (Rabbit)  DataSheet

BAM-63-151-EX

50 UL ¥30,000
$400

Anti Mrc1 Schizosaccharomyces pombe  (Rabbit)  DataSheet

BAM-63-153-EX

250 UL N/A

Product Name Cat# Quantity Price

Anti Dnmt1 (1-248), Mouse (Rabbit) Unlabeled DataSheet

BAM-70-201-EX

50 UG ¥26,300
$351

Anti Dnmt1 (1037-1386), Mouse (Rabbit) Unlabeled DataSheet

BAM-70-203-EX

50 UG ¥29,000
$387

Anti HP1 (α) /CBX5, Human (Rabbit)  DataSheet

BAM-70-221-EX

50 UG ¥21,000
$280

Anti HP1 (β)/CBX1, Human (Rabbit)  DataSheet

BAM-70-223-EX

50 UG ¥21,000
$280

Anti HP1 (γ)/CBX3, Human (Rabbit)  DataSheet

BAM-70-225-EX

50 UG ¥21,000
$280

Anti 5-Methylcytosine (Mouse)  DataSheet

BAM-51-003-EX

100 UG ¥34,500
$460

Product Name Cat# Quantity Price

Anti Rpn3 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-201-EX

100 UL ¥40,000
$534

Anti Rpn5 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-203-EX

100 UL ¥40,000
$534

Anti Rpn7 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-205-EX

100 UL ¥40,000
$534

Anti Rpn9 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-207-EX

100 UL ¥42,000
$560

Anti Rpn12 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-209EX

50 UL ¥30,000
$400

Anti Nob1 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-211-EX

100 UL ¥42,000
$560

Anti Nas6/p28 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-213-EX

100 UL ¥42,000
$560

Anti Tem1 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-215-EX

100 UL ¥42,000
$560

“Anti HP1Beta/CBX1 polyclonal antibody” also belongs to the following categories.