Anti HP1ƒÁ/CBX3 polyclonal antibody


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Background

Heterochromatin protein 1 (HP1) is a major component of heterochromatin play a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionarily conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family of proteins is encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1γ is encoded by the Chromobox homolog 3 (CBX3) gene. HP1γhas been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (Ref 1).
The product is prepared by immunizing rabbit with the synthetic peptide WHSCPEDEAQ-C corresponding to the C-terminal sequence of human HP1γ(Ref 2, 3) and by affinity purification with the peptide. The antiserum preparation has been directed by Prof. T. Haraguchi.

Application

1) Western blotting (1/2000~1/10000)
2) Immunofluoresece staining
3) Chromatin immunoprecipitation (ChIP) (Ref. 3)


Western blotting.
Sample:Baby Hamster Kidney cells.

Immunofluorescent staining
Sample : Baby Hamster Kidney cells
Cells were fixed with para-formaldehyde.
The second antibody F Alexa Fluor 594- conjugated goat anti-rabbit IgG antibody

<Reference>
1. Lomberk G et al. Genome Biol. 7, 228 (2006), review
2. Kametaka A et al. Genes Cells 7, 1231 (2002)
3. Wang F et al. Mol. Cell. Biol. 26, 4028 (2006)

Product List

Product Name Cat# Quantity Price

Anti HP1 (γ)/CBX3, Human (Rabbit)  DataSheet

BAM-70-225-EX

50 UG ¥21,000
$280

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Anti Dis1 Schizosaccharomyces pombe  (Rabbit) Unlabeled

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Anti DIS3 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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Anti Pad Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

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100 UL ¥35,000
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Anti Ppa2 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

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100 UL ¥35,000
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100 UL ¥35,000
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Anti Mrc1 Schizosaccharomyces pombe  (Rabbit)  DataSheet

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Anti Mrc1 Schizosaccharomyces pombe  (Rabbit)  DataSheet

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250 UL N/A

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Anti Dnmt1 (1-248), Mouse (Rabbit) Unlabeled DataSheet

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Anti HP1 (α) /CBX5, Human (Rabbit)  DataSheet

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Anti HP1 (β)/CBX1, Human (Rabbit)  DataSheet

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50 UG ¥21,000
$280

Anti HP1 (γ)/CBX3, Human (Rabbit)  DataSheet

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50 UG ¥21,000
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100 UL ¥40,000
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Anti Rpn7 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥40,000
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Anti Rpn9 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥42,000
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Anti Rpn12 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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Anti Nob1 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥42,000
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Anti Nas6/p28 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥42,000
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Anti Tem1 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥42,000
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