Anti U1 snRNP C (U1C) monoclonal antibody


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Background

The spliceosomal U1C protein is critical to the initiation and regulation of precursor messenger RNA (pre-mRNA) splicing, as part of the U1 small nuclear ribonucleoprotein particle (snRNP). U1C is needed for efficient complex formation of U1 snRNP with a 5’ splice site.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Data Link Swiss-Prot Q62241

Application

1. Western blotting
2. Immunocytochemistry
3. Immunohistochemistry (frozen section)
4. ELISA
    Other applications are not tested.


< Immunofluorescent staining >
Sample : L929 (mouse) cells


<Western blotting>
Sample : U1C protein in HeLa total cell extract


< Immunoprecipitation >
lane1 : no extract
lane2 : U1C protein in HeLa total cell extract
HC, LCGIgG heavy & light chain

<Reference>
1. Muto,Y. et al. (2004) "The structure and biochemical properties of the human spliceosomal protein U1C." J Mol Biol 341: 185–198 PMID : 15312772
2. Pomeranz Krummel, D.A. et al. (2009) “Crystal structure of human spliceosomal U1 snRNP at 5.5A resolution.” Nature 458: 475-480 PMID : 19325628

Product List

Product Name Cat# Quantity Price

Anti U1 snRNP C (U1C), Mouse (Rat) Unlabeled DataSheet

BAM-70-400-EX

100 UG ¥35,000
$467

Species:MS
Host:RAT
Clone:4H12
Application:WB/IHC(f)/ELISA/IC

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