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Reagents for Genetic Engineerring

Taq DNA Polymerase (+dNTPs), with Standard buffer


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Background

Thermus aquaticus DNA polymerase (Taq DNA polymerase) was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa..

• This enzyme is suitable for PCR reactions; capable of amplifying DNA with various primers.

Application

1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine) at the 3’-blunt ends

*Use of excess amount is not recommended
General composition of PCR reaction mixture (total 50µl)
Taq DNA polymerase (5 units/μl) *0.25 μl
10 x Reaction Buffer (Taq) 5 μl
2.5 mM (each) dNTPs 4 μl
Template <500ng
Primer 1 0.2`1.0μM (final conc.)
Primer 2 0.2`1.0μM (final conc.)
Sterile distilled water up to 50μl

 

< SDS-PAGE of Taq DNA polymerase >

 

< SDS-PAGE of Taq DNA polymerase >

PCR condition
98°C  10sec
57°C  30sec@@25cycles
72°C@8min
(2min in the case of 2kb DNA)

Product List

Product Name Cat# Quantity Price

Taq DNA polymerase (+dNTPs), with Standard buffer DataSheet ,

BAM-02-001-EX

200 UNIT ¥5,100
$68

Taq DNA polymerase (+dNTPs), with Standard buffer DataSheet ,

BAM-02-001-5EX

5*200 UNIT ¥20,400
$272

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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