useful for measurement the amount of HIV-1 Gag p24 antigen

HIV-1 p24 ELISA Kit


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Background

This kit can measure the amount of HIV-1 Gag p24 antigen in cell culture medium handily by a sandwich ELISA (Enzyme Linked Immunosorbent Assay) method.
p24 antigen is a structure protein of HIV-1, so when this is measured, it's possible to presume the virus amount in the sample

Principle

This kit is made based on the principle of a sandwich ELISA method using an antibody coated plate as a solid phase, a biotinyl antibody and a peroxidase-labeled streptavidin

Feature

¥ p24 of subtype AE can be measured at the same sensitivity as subtype B, because affinity purified polyclonal antibody raised against the full length recombinant p24 is used.
¥ Risk-free kit, because neither patient sera nor active virus products are used.
¥ Assay can be performed at room temperature


Standard curve of HIV-1 p24 measurement by the 2 hour assay.

 

<Preservation and Expiration Date>
Storage :  2 – 8°C (Please don't freeze.)
Expiration date for use :  1 year

<Required Reagent, Apparatus and Equipment>
1. Deionized water
2. Test tubes or microtubes (for sample preparation)
3. Micropipettes and tips
4. Microplate reader

<Reference>
1. White, E. L., et al., J. Virol. Methods 70: 113-115 (1998)

Product List

Product Name Cat# Quantity Price

HIV-1 p24 ELISA DataSheet ,

BIA-80-001-EX

96 ASSAY N/A

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