Anti Autophagy antibody


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Background

Autophagy is the primary means for the degradation of cytoplasmic constituents in the lysosome and has been considered to be an important defensive mechanism during starvation. Three distinct types of autophagy have been identified, macroautophagy, microautophagy and chaperone-mediated autophagy, and, among these, the molecular mechanism and physiologic significance of macroautophagy have been best studied. Macroautophagy (usually referred to simply as autophagy) is mediated by the autophagosome, a unique and specialized cellular organelle. Atg5, Atg12 and Atg16L form a 800-kD protein complex in the cytosol. Almost all Atg5 and Atg12 are covalently attached to each other and present as an Atg12-Atg5 conjugate within cells. A small fraction of the Atg12-Atg5·Atg16L complex targets to the outer side of isolation membrane (or phagophore), which is an intermediate structure during autophagosome formation. The protein complex plays an essential role in the membrane elongation and dissociate from the membrane upon completion of autophagosome formation. Recent Atg5 gene knockout studies have suggested that autophagy is important for a variety of physiological processes such as starvation adaptation during food withdrawal or after birth, intracellular protein quality control and killing of intracellular pathogens.


Fig.1 Intracellular protein degradation systems
Macroautophagy. A small volume of cytoplasm is enclosed by the autophagic isolation membrane, which eventually results in the formation of an autophagosome. The outer membrane of the autophagosome then fuses with the lysosome where the cytoplasm-derived materials are degraded.


Fig.2. Total cell lysates were prepared from wild-type (Atg5+/+) and Atg5-/- mouse embryonic fibroblasts and subjected to immunoblotting with anti-Atg5 antibody (1:2000). Note that almost all Atg5 is conjugated with Atg12 and Atg5 monomer is not detected.

Fig.3 Total cell lysates were prepared from Atg5+/+ (WT) and Atg5-/- (KO) mouse embryonic fibroblasts and subjected to immunoblotting with anti-Atg12 antibody (1:2000). Note that almost all Atg12 is conjugated with Atg5 and Atg12
Product List

Anti Atg5, CAC-TMD-PH-AT5, Species : HU, Host : RAB, Application : WB
Anti Atg 12, CAC-TMD-PH-AT12, Species : MS, Host : RAB, Application : WB/IHC
Anti Atg 16, LCAC-TMD-PH-AT16L, Species : MS, Host : RAB, Application : WB/IHC


Product Name Cat# Quantity Price

Anti Atg5, Human (Rabbit)  DataSheet

CAC-TMD-PH-AT5

100 UL N/A

Anti Atg12, Mouse (Rabbit)  DataSheet

CAC-TMD-PH-AT12

100 UL N/A

Anti Atg16L, Mouse (Rabbit)  DataSheet

CAC-TMD-PH-AT16L

100 UL N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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