Anti LC3 monoclonal antibody


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Background

LC3B is one of the mammalian Atg8 homologs and widely used as an autophagosome marker. Immediately after synthesis, LC3 is processed by Atg4 and becomes LC3-I. Upon induction of autophagy, the C-terminal glycine of LC3-I is conjugated to phosphatidylethanolamine, resulting in formation of membrane-bound LC3-II. Most LC3-II is thought be present on autophagosome membrane. The autophagosome subsequently fuses with a lysosome, where inside materials, including LC3-II, are degraded. The expression level of LC3-II generally correlates with the number of autophagosome

Application

Western Blot: 1/500-1/1,000.
Predicted molecular weight: LC3-I is 18 kDa and LC3-II is 16 kDa

Optimum dilution rate should be determined by end users

References

1. Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000)
2. Mizushima, N., Yoshimori, T. How to interpret LC3 immunoblotting Autophagy 3:542-545 (2007)

Product List

Product Name Cat# Quantity Price

Anti LC3, Human (Mouse) Unlabeled DataSheet

CAC-CTB-LC3-1-50

50 UG ¥50,000
$667

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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