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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti ƒÀ-Actin[PS1TP5BP1] monoclonal antibody (clone : ACTBD11B7)


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Background

Beta actin is one of six different actin isoforms which have been identified. ACTB is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of ACTB by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. ACTBD11B7-2]

Western_blot_protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ACTB mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG.

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ACTB mAb. [Lot No. ACTBD11B7-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti ACTB, Human (Mouse) Unlabeled DataSheet

CBX-CBX00270

100 UG ¥39,000
$520

“Anti ƒÀ-Actin[PS1TP5BP1] monoclonal antibody (clone : ACTBD11B7)” also belongs to the following categories.