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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti Twist[SCS,ACS3,BPES2,BPES3,TWIST1] monoclonal antibody (clone : Twist2C1a)


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Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of TWIST by Western blot. Samples: Whole cell lysate from human A2058 (H, 25 μg), mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. Twist2C1a-1]

Predicted molecular weight: 20 kDa

Western_blot_protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TWIST mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. Twist2C1a-1]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TWIST mAb. [Lot No. Twist2C1a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti TWIST, Human (Mouse) Unlabeled DataSheet

CBX-CBX00383

100 UG ¥39,000
$520

“Anti Twist[SCS,ACS3,BPES2,BPES3,TWIST1] monoclonal antibody (clone : Twist2C1a)” also belongs to the following categories.