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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti Stat3 [APRF, FLJ20882, MGC16063] monoclonal antibody (clone : STAAD22A)


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Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of STAT3 by Western blot. Samples: Whole cell lysate from human HeLa (H, 25 μg) , mouse NIH3T3 (M, 50 μg) and rat F2408 (R, 50 μg) cells. [Lot No. STAAD22A-2]

Predicted molecular weight: 87 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
RIPA lysate of HeLa cells was incubated with anti-STAT3 mAb. [Lot No. STAAD22A-2]

Predicted molecular weight: 87 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-STAT3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. STAAD22A-2]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STAT3 mAb. [Lot No. STAAD22A-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti STAT3, Human (Mouse) Unlabeled DataSheet

CBX-CBX00237

100 UG ¥39,000
$520

“Anti Stat3 [APRF, FLJ20882, MGC16063] monoclonal antibody (clone : STAAD22A)” also belongs to the following categories.