• HOME
  • Products
  • Anti CDC5L [CEF1, hCDC5, PCDC5RP, KIAA0432, dJ319D22.1] monoclonal antibody (clone : 2136C1a)

Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti CDC5L [CEF1, hCDC5, PCDC5RP, KIAA0432, dJ319D22.1] monoclonal antibody (clone : 2136C1a)


print

Background

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of CDC5L by Western blot. Samples: Whole cell lysate from human HeLa (H, 50 μg), mouse NIH3T3 (M, 50 μg) and rat F2408 (R, 50 μg) cells. [Lot No. 2136C1a-1]

Predicted molecular weight: 92 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CDC5L mAb. [Lot No. 2136C1a-1]

Predicted molecular weight: 92 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDC5L mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2136C1a-1]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDC5L mAb. [Lot No. 2136C1a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti CDC5L, Human (Mouse) Unlabeled DataSheet

CBX-CBX00358

100 UG ¥39,000
$520

“Anti CDC5L [CEF1, hCDC5, PCDC5RP, KIAA0432, dJ319D22.1] monoclonal antibody (clone : 2136C1a)” also belongs to the following categories.