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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti CDK7 [CAK1, STK1,CDKN7, p39MO15] monoclonal antibody (clone : 2141C4a)


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Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of human CDK7 by Western blot. Samples: Whole cell lysate (50 μg) from HeLa cells. [Lot No. 2141C4a-1]

Predicted molecular weight: 39 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CDK7 mAb. [Lot No. 2141C4a-1]

Predicted molecular weight: 39 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDK7 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2141C4a-1]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDK7 mAb. [Lot No. 2141C4a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti CDK7, Human (Mouse) Unlabeled DataSheet

CBX-CBX00360

100 UG ¥39,000
$520

“Anti CDK7 [CAK1, STK1,CDKN7, p39MO15] monoclonal antibody (clone : 2141C4a)” also belongs to the following categories.