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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti CUL3 monoclonal antibody (clone : 2236C1a)


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Background

Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY. [UniProtKB@Function]

Application

[Western Blot]
Detection of CUL3 by Western blot. Samples: Whole cell lysate from human HeLa (H, 50 μg) , mouse NIH3T3 (M, 50 μg) and rat F2408 (R, 50 μg) cells. [Lot No. 2236C1a-1]

Predicted molecular weight: 88 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CUL3 mAb. [Lot No. 2236C1a-1]

Predicted molecular weight: 88 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CUL3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2236C1a-1]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CUL3 mAb. [Lot No. 2236C1a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti CUL3, Human (Mouse) Unlabeled DataSheet

CBX-CBX00382

100 UG ¥39,000
$520

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