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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti RPA32 [RPA2, REPA2] monoclonal antibody (clone : 2927C5)


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Background

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. [UniProtKB Function]

Application

[Western Blot]
Detection of human RPA2 by Western blot. Samples: Whole cell lysate (25 μg) from HeLa cells. [Lot No. 2927C5-1]

Predicted molecular weight: 29 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-RPA2 mAb. [Lot No. 2927C5-1]

Predicted molecular weight: 29 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RPA2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2927C5-1]

Flow cytometry protocolPDF

 

[Flow cytometry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RPA2 mAb. [Lot No. 2927C5-1]

Flow cytometry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti RPA2, Human (Mouse) Unlabeled DataSheet

CBX-CBX00427

100 UG ¥39,000
$520

“Anti RPA32 [RPA2, REPA2] monoclonal antibody (clone : 2927C5)” also belongs to the following categories.