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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti SP1 monoclonal antibody (clone : SP15H09)


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Application

[Western Blot]
Detection of human SP1 by Western blot. Samples: Whole cell lysate (50 μg) from HeLa cells. [Lot No. SP15H09-2]

Predicted molecular weight: 80 kDa

Western_blot_protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-SP1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. SP15H09-2]

Flow cytometry protocolPDF

 

[Flow cytometry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SP1 mAb. [Lot No. SP15H09-2]

Flow cytometry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti SP1, Human (Mouse) Unlabeled DataSheet

CBX-CBX00145

100 UG ¥39,000
$520

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