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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti BRD3 [ORFX, RING3L, KIAA0043] monoclonal antibody (clone : 2088C3a)


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Background

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of BRD3 by Western blot. Samples: Whole cell lysate from human HeLa (H, 50 μg) , mouse NIH3T3 (M, 50 μg) and rat F2408 (R, 50 μg) cells. [Lot No. 2088C3a-1]

Predicted molecular weight: 79 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BRD3 mAb. [Lot No. 2088C3a-1]

Predicted molecular weight: 79 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRD3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2088C3a-1]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRD3 mAb. [Lot No. 2088C3a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti BRD3, Human (Mouse) Unlabeled DataSheet

CBX-CBX00328

100 UG ¥39,000
$520

“Anti BRD3 [ORFX, RING3L, KIAA0043] monoclonal antibody (clone : 2088C3a)” also belongs to the following categories.