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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti CARD8 [DACAR, NDPP1, TUCAN, CARDINAL, KIAA0955, MGC57162, DKFZp779L0366] monoclonal antibody (clone : 2088C3a)


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Background

Caspase recruitment domain (CARD)-containing proteins, such as CARD8, are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB; see MIM 164011) in the context of apoptosis or inflammation, respectively (Bouchier-Hayes et al., 2001).[supplied by OMIM]. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of human CARD8 by Western blot. Samples: Whole cell lysate (50 μg) from HeLa cells. [Lot No. 2108C2a-1]

Predicted molecular weight: 48 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CARD8 mAb. [Lot No. 2108C2a-1]

Predicted molecular weight: 48 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CARD8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2108C2a-1]

Flow cytometry protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CARD8 mAb. [Lot No. 2108C2a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti CARD8, Human (Mouse) Unlabeled DataSheet

CBX-CBX00332

100 UG ¥39,000
$520

“Anti CARD8 [DACAR, NDPP1, TUCAN, CARDINAL, KIAA0955, MGC57162, DKFZp779L0366] monoclonal antibody (clone : 2088C3a)” also belongs to the following categories.