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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti MARS/Methionyl tRNA synthetase [MTRNS, METRS] monoclonal antibody (clone : MARSD10B4)


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Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of MARS by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. MARSD10B4-3]

Predicted molecular weight: 101 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-MARS mAb. [Lot No. MARSD10B4-2]

Predicted molecular weight: 101 kDa

Immunoprecipitation protocolPDF

 

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-MARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. MARSD10B4-3]

Flow cytometry protocolPDF

 

[Flow cytometry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MARS mAb. [Lot No. MARSD10B4-2]

Flow cytometry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti MARS, Human (Mouse) Unlabeled DataSheet

CBX-CBX00271

100 UG ¥39,000
$520

“Anti MARS/Methionyl tRNA synthetase [MTRNS, METRS] monoclonal antibody (clone : MARSD10B4)” also belongs to the following categories.