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This immunoassay kit allows for the in vitro quantitative determination of bovine CPT II concentrations in cell culture supernates, serum, plasma and other biological fluids.

Bovine carnitine palmitoyltransferase II [CPT II)] ELISA Kit


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Background

Carnitine palmitoyltransferase II, also known as CPT2, is a human gene. Carnitine palmitoyltransferase II precursor (CPT2) is a nuclear protein which is transported to the mitochondrial inner membrane. CPT2 together with carnitine palmitoyltransferase I oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders and carnitine palmitoyltransferase II deficiency.

Feature and Advantages

<PRINCIPLE OF THE ASSAY>
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPT II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CPT II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CPT II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPT II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

< SPECIFICITY>
This assay recognizes recombinant and natural bovine CPT II. No significant cross-reactivity or interference was observed.

<DETECTION RANGE>
1.56 ng/ml-100 ng/ml.
The standard curve concentrations used for the ELISA’s were 100 ng/ml, 50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml.

<SENSITIVITY>
This assay recognizes recombinant and natural bovine CPT II. No significant cross-reactivity or interference was observed.

Kit component

  Reagent Quantity
1. Assay plate 1
2. Standard 2
3. Sample Diluent 1 x 20 ml
4. Biotin-antibody Diluent 1 x 10 ml
5. HRP-avidin Diluent 1 x 10 ml
6. Biotin-antibodyt 1 x 120μl
7. HRP-avidin 1 x 120μl
8. Wash Buffer 1 x 20 ml (25×concentrate)
9. TMB Substrate 1 x 10 ml
10. Stop Solution 1 x 10 ml
Product List

Product Name Cat# Quantity Price

Species : 1.56 ng/ml -100 ng/ml
Assay range : 100 µL (Culture Supernatant, Plasma, Serum)
Assay sample volume : 6-8 hr.+3 hr

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