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This assay recognizes recombinant and natural rat Kim-1.

Rat kidney injury molecule 1 (Kim-1) ELISA Kit


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Background

Kim-1 (kidney injury molecule-1) is a type I transmembrane glycoprotein expressed on dedifferentiated renal proximal tubule epithelial cells undergoing regeneration after toxic or ischemic injury. The extracellular domain of Kim-1 is composed of an immunoglobulin-like domain topping a long mucin-like domain, a structure that points to a possible role in cell adhesion by homology to several known adhesion proteins. Two splice variants (a and b), of the human Kim-1 having identical extracellular domains, differ in their cytoplasmic domains and tissue distributions.

Feature and Advantages

<PRINCIPLE OF THE ASSAY>
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kim-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Kim-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Kim-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Kim-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

< SPECIFICITY>
This assay recognizes recombinant and natural rat Kim-1.@No significant cross-reactivity or interference was observed.

<DETECTION RANGE>
312 pg/ml-20000 pg/ml
The standard curve concentrations used for the ELISA’s were 20000 pg/ml, 10000 pg/ml, 5000 pg/ml, 2500 pg/ml, 1250 pg/ml, 625 pg/ml, 312 pg/ml.

<SENSITIVITY>
The minimum detectable dose of rat Kim-1 is typically less than 78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the

Kit component

  Reagent Quantity
1. Assay plate 1
2. Standard 2
3. Sample Diluent 1 x 20 ml
4. Biotin-antibody Diluent 1 x 10 ml
5. HRP-avidin Diluent 1 x 10 ml
6. Biotin-antibodyt 1 x 120μl
7. HRP-avidin 1 x 120μl
8. Wash Buffer 1 x 20 ml (25×concentrate)
9. TMB Substrate 1 x 10 ml
10. Stop Solution 1 x 10 ml
Product List

Product Name Cat# Quantity Price

CSBE08808R
Species : 312 pg/ml - 20000 pg/ml
Assay range : 100 µL(Cell Culture Supernates, Plasma,Serum)
Assay sample volume : 6-8 hr.{3 hr

CSBE08809M

Species : 312 pg/ml - 20000 pg/ml
Assay range : 100 µL(Cell Culture Supernates, Plasma,Serum)
Assay sample volume : 6-8 hr.{3 hr

CSBE08807H
Species : 0.625 ng/ml - 40 ng/ml.
Assay range : 100 µL(Cell Culture Supernates, Plasma,Serum)
Assay sample volume : 6-8 hr.{3 hr


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