This immunoassay kit allows for the in vitro quantitative determination of rat NGF concentrations in cell culture supernates, serum, plasma and other biological fluids.

Rat Nerve Growth Factor (NGF) ELISA Kit


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Background

Nerve growth factor (NGF), is a small secreted protein which induces the differentiation and survival of particular target neurons (nerve cells).NGF is a protein that consists of 3 types of polypeptide chains -- alpha, beta and gamma -- that interact to form the protein. The NGF beta chain (NGFB) is solely responsible for the nerve growth stimulating activity of NGF. The NGFB gene is in chromosome band 1p22. An ointment containing NGF has been reported to help heal bedsores (decubiti).
NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There are some data that show that NGF can be transported from the axon tip to soma, but it is unclear if this is necessary for effective cell signalling; in fact there are data showing that it is not. What is clear is that NGF binding and activation of TrkA is required for NGF-mediated neuronal survival and differentiation. NGF binds at least two receptors on the surface of cells which are capable of responding to this growth factor, TrkA and the LNGFR.
In 2005, Italian scientists at University of Pavia found that a protein molecule known as the nerve growth factor (NGF) has high levels when people first fall in love, but these levels return to as they were after one year. Specifically, four neurotrophin levels, i.e. NGF, BDNF, NT-3, and NT-4, of 58 subjects who had recently fallen in love were compared with levels in a control group who were either single or already engaged in a long-term relationship. The results showed that NGF levels were significantly higher in the subjects in love than as compared to either of the control groups. It has also been tied to Alzheimer's disease.

Feature and Advantages

<PRINCIPLE OF THE ASSAY>
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

< SPECIFICITY>
This assay recognizes recombinant and natural rat NGF. No significant cross-reactivity or interference was observed.

<DETECTION RANGE>
0.156 ng/ml-10 ng/ml.
The standard curve concentrations used for the ELISA’s were
10 ng/ml, 5 ng/ml, 2.5 ng/ml, 1.25 ng/ml, 0.625 ng/ml, 0.312 ng/ml, 0.156 ng/ml.

<SENSITIVITY>
The minimum detectable dose of rat NGF is typically less than 0.04 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.

Kit component


Reagent Quantity
1. Assay plate 1
2. Standard 2
3. Sample Diluent 1 x 20 ml
4. Biotin-antibody Diluent 1 x 10 ml
5. HRP-avidin Diluent 1 x 10 ml
6. Biotin-antibodyt 1 x 120μl
7. HRP-avidin 1 x 120μl
8. Wash Buffer 1 x 20 ml (25×concentrate)
9. TMB Substrate 1 x 10 ml
10. Stop Solution 1 x 10 ml
Product List

Product Name Cat# Quantity Price

Species : 0.156 ng/ml -10 ng/ml
Assay range : 100 µL(Cell Culture Supernates, Plasma,Serum)
Assay sample volume : 6-8 hr.{3 hr


Product Name Cat# Quantity Price

Anti Vitellogenin, Killifish (Mouse) Unlabeled DataSheet

KAL-KH006

100 UG ¥40,000
$534

Anti Vitellogenin : F10-2, Killifish (Mouse) Unlabeled DataSheet

EBT-AVMF10-2-EX

1 ML ¥50,000
$667

Anti Vitellogenin : D3-3, Killifish (Mouse) Unlabeled DataSheet

EBT-AVMD3-3-EX

1 ML ¥50,000
$667

Anti Vitellogenin, Killifish (Mouse) Horseradish Peroxidase DataSheet

EBT-MVD33-HRP-EX

0.5 ML ¥50,000
$667

Anti Vitellogenin, Fish (Mouse) Unlabeled DataSheet

KAL-KH005

100 UG ¥40,000
$534

Anti Vitellogenin : B6-1, - (Mouse) Unlabeled DataSheet

EBT-MUMB6-1-EX

1 ML N/A

Anti Vitellogenin : F61-2, - (Mouse) Unlabeled DataSheet

EBT-MUMF61-2-EX

1 ML N/A

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