This This immunoassay kit allows for the in vitro quantitative determination of mouse IL-8 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Mouse Interleukin 8 (IL-8)ELISA Kit


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Background

Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. There are more receptors of the surface membrane capable to bind IL-8. The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Primary function of IL-8 is the induction of chemotaxis in its target cells (e.g. neutrophil granulocytes). In neutrophils series of cell-physiological responses required for migration and its target function phagocytosis are also induced like increase of intracellular Ca2+, exocytosis (e.g. histamine release), respiratory burst. IL-8 can be secreted by any cells with toll-like receptors which are involved in the innate immune response. IL-8's primary function is to recruit neutrophils to phagocytose the antigen which trigger the antigen pattern toll-like receptors. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.

Feature and Advantages

<PRINCIPLE OF THE ASSAY>
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

< SPECIFICITY>
This assay recognizes recombinant and natural mouse IL-8. No significant cross-reactivity or interference was observed.

<DETECTION RANGE>
31.2 pg/ml-2000 pg/ml.  The standard curve concentrations used for the ELISA’s were  2000pg/ml, 1000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml.

<SENSITIVITY>
The minimum detectable dose of mouse IL-8 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.

Kit component

  Reagent Quantity
1. Assay plate 1
2. Standard 2
3. Sample Diluent 1 x 20 ml
4. Biotin-antibody Diluent 1 x 10 ml
5. HRP-avidin Diluent 1 x 10 ml
6. Biotin-antibodyt 1 x 120μl
7. HRP-avidin 1 x 120μl
8. Wash Buffer 1 x 20 ml (25×concentrate)
9. TMB Substrate 1 x 10 ml
10. Stop Solution 1 x 10 ml
Product List

Product Name Cat# Quantity Price

CSB-CSB-E07274M
Species : MS
Assay range : 31.2 pg/ml-2000 pg/ml.
Assay sample volume : 100 L (Cell Culture Supernates,Plasma,Serum)
Assay time : 6-8 hr.{3 hr

CSB-CSB-E07273R
Species : RAT
Assay range : 18.75 pg/ml-1200 pg/ml.
Assay sample volume : 100 L (Cell Culture Supernates,Plasma,Serum)
Assay time : 6-8 hr.{3 hr

CSB-CSB-E06905RB
Species : RAB
Assay range : 23.5 pg/ml-1500 pg/ml.
Assay sample volume : 100 L (Cell Culture Supernates,Plasma,Serum)
Assay time : 6-8 hr.{3 hr

CSB-CSB-E06787P
Species : POR
Assay range : 156 pg/ml-10000 pg/ml..
Assay sample volume : 100 L (Cell Culture Supernates,Plasma,Serum)
Assay time : 6-8 hr.{3 hr

CSB-CSB-E04641H
Species : HU
Assay range : -
Assay sample volume : -
Assay time : -

CSB-CSB-E12877FH
Species : Fish
Assay range : -
Assay sample volume : -
Assay time : -

CSB-CSB-E08051MO
Species : MKY
Assay range : -
Assay sample volume : -
Assay time : -

CSB-CSB-E08052C
Species : CAN
Assay range : -
Assay sample volume : -
Assay time : -

CSB-CSB-E08901DU
Species : Duck
Assay range : -
Assay sample volume : -
Assay time : -


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