useful for hyaluronan research

Fluoresceinamine labeled Sodium Hyaluronate


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Background

Hyaluronan (HA) is a glycosaminoglycan composed of repeating disaccharide units of N-acetyl-D-glucosamine (GlcNAc) and D-glucuronic acid (GlcUA). HA is abundant in synovial fluid, skin, umbilical cord, and vitreous body exists as unbranched polysaccharide chains. This product is prepared by the fluorescent labeling of HA according to the method of Ogamo et.al.1). Fluoresceinamine molecules are chemically attached to carboxyl groups of the GlcUA of HA. This solution is dissolved in PBS (|) and sterilized by filtration. The endotoxin content is in accordance with the product specifications. The excitation wavelength is 490`500 nm and the emission wavelength is 515`525 nm. The Certification of Analysis lists actual values for product specifications.


Fluoresce inamine labeled Sodium Hyaluronate flyer download [PDF]


Fluorescence photograph of rat knee joint after intra-articular injection of FAHA-M1

Specification

  specification
Appearanc yellow green solution or yellow green lyophilizate
Concentration of FAHA
(PBS(-) solution)
0.90`1.10mg/mL
Mw calculated
by Intrinsic Viscosity
1211×103`1603×103 dalton (FAHA-H1,H2)
602×103`1118×103 dalton (FAHA-M1,M2)
102×103`304×103 dalton (FAHA-L1,L2)
40×103`80×103 dalton (FAHA-S1)
20×103`30×103 dalton (FAHA-T1)
Wavelength of Excitation 490`500 nm
Wavelength of Emission 515`525 nm
Degree of Substitution* 0.600`1.100%
Fluorescent Purity
on Electrophoresis
one band at same mobility with original NaHA
Concentration of Endotoxin
(PBS(-) solution)
ƒ0.25 EU/mL
Storage Store protected from light at -20°C or below. We recommend storing in aliquots appropriate for anticipated usage.

NaHA : Sodium Hyaluronate

*Degree of Substitution was expressed as the molar percentage of bound fluoresceinamine groups to disaccharide units.


Excitation and emission spectrum of FAHA (pH10)


Elution profiles of FAHA with various Mw on GPC
(Column: TSKgel G6000PWXL (7.8mmI.D. × 30cm × 2))


Correlation between Mw of FAHA and elution volume
(The formula is expressed as a linear regression equation. R means a correlation coefficient.)


Cellulose acetate membrane electrophoresis of FAHA-L1
NaHA : Sodium Hyaluronate

 

<Reference>
1. Ogamo, A., et.al.: Carbohydr. Res., 105, 69 (1982)

Product List

Product Name Cat# Quantity Price

Fluoresceinamine Labeled Sodium Hyaluronate (H1), Label DataSheet

CSR-FAHA-H1

3 ML ¥66,000
$880

Fluoresceinamine Labeled Sodium Hyaluronate (M1), Label DataSheet

CSR-FAHA-M1

3 ML ¥66,000
$880

Fluoresceinamine Labeled Sodium Hyaluronate (L1), Label DataSheet

CSR-FAHA-L1

3 ML ¥66,000
$880

Fluoresceinamine Labeled Sodium Hyaluronate (S1), Label DataSheet

CSR-FAHA-S1

3 ML ¥66,000
$880

Fluoresceinamine Labeled Sodium Hyaluronate (T1), Label DataSheet

CSR-FAHA-T1

3 ML ¥66,000
$880

Product Name Cat# Quantity Price

Fluoresceinamine Labeled Sodium Hyaluronate (H2), Label DataSheet

CSR-FAHA-H2

3 MG ¥55,000
$734

Fluoresceinamine Labeled Sodium Hyaluronate (M2), Label DataSheet

CSR-FAHA-M2

3 MG ¥55,000
$734

Fluoresceinamine Labeled Sodium Hyaluronate (L2), Label DataSheet

CSR-FAHA-L2

3 MG ¥55,000
$734

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