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useful for research of dermatan sulfate

Fluoresceinamine labeled Sodium Dermatan Sulfate


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Background

Dermatan sulfate (DS) is a sulfated glycosaminoglycan composed of repeating disaccharide units of N-acetyl-D-galactosamine (GalNAc) and D-glucuronic acid (GlcUA) or D-iduronic acid (IdoUA). DS is abundant in skin and exists as unbranched polysaccharide chains covalently linked to the protein core of proteoglycans. This product is prepared by the fluorescent labeling of DS derived from porcine skin according to the method of Ogamo et.al.1). Fluoresceinamine molecules are chemically attached to carboxyl groups of the GlcUA or IdoUA of DS. FADS-B1 contains approximately 80 % of disaccharide units with one sulfate groups as 4-O-sulfation of GalNAc. This solution is dissolved in PBS (-) and sterilized by filtration. The endotoxin content is in accordance with the product specifications. The excitation wavelength is 490~500 nm and the emission wavelength is 515~525 nm. The enclosed Certification of Analysis lists actual values for product specifications.


Fluoresce inamine labeled Sodium Hyaluronate flyer download [PDF]


Constitutional formula of Sodium Dermatan Sulfate


Analysis of binding activities with BMP-4 by fluorescence correlation spectroscopy (FCS)

Fluorescence correlation spectroscopy (FCS) is an analytical method of molecular - association or dissociation with one molecular level. The interaction of molecules can be detected as a prolongation of average diffusion time (DT) derived from a fluorescent molecule. The figure shows the effect of recombinant human bone morphogenetic protein-4 (rhBMP-4) on the DT of various fluoresceinamine-labeled GAGs. The result indicates relatively strong interactions between rhBMP-4 and FACS-E1, FACS-P1 or FAHep-N1. (J Cell Physiol, 2008, 217(3):769-777)

Specification

  Specification
Appearance yellow green solution
Concentration of FADS 0.90`1.10mg/mL
Wavelength of Excitation 490`500 nm
Wavelength of Emission 515`525 nm
Degree of Substitution* 0.600`1.100%
Fluorescent Purity
on Electrophoresis
one band at same mobility with original NaDS
Concentration of Endotoxin ƒ0.25 EU/mL
Storage Store protected from light at -20°C or below. We recommend storing in aliquots appropriate for anticipated usage.

NaDS: Sodium Dermatan Sulfate

*Degree of Substitution was expressed as the molar percentage of bound fluoresceinamine groups to disaccharide units.


Excitation and emission spectrum of FADS-B1 (pH10)


Elution profiles of FADS-B1, FAHep-N1 and FAHS-P1 on GPC
(Column: TSKgel G4000PWXL (7.8mmI.D. × 30cm × 1))


Cellulose acetate membrane electrophoresis of FADS-B1
NaDS: Sodium Dermatan Sulfate

Raw materials of FAHA, FACS, FADS, FAHep and FAHS
Product Name Raw Material Product Name Raw Material
FAHA-H1, H2 Streptococcus sp. FACS-A1 whale cartilage
FAHA-M1, M2 chicken comb FACS-C1 shark cartilage
FAHA-L1, L2 Streptococcus sp. FACS-D1 shark cartilage
FAHA-S1 Streptococcus sp. FACS-E1 squid cartilage
FAHA-T1 Streptococcus sp. FADS-B1 porcine skin
FAHep-N1 porcine intestine FAHS-P1 porcine kidney

 

<References>
1. Ogamo A et.al.: Carbohydr.Res., 105, 69 (1982)

Product List

Product Name Cat# Quantity Price

Fluoresceinamine Labeled Sodium Dermatan Sulfate (B1), Label DataSheet

CSR-FADS-B1

3 ML ¥66,000
$880

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