Anti Osteopontin (OPN)


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Background

Osteopontin (Early T-Lymphocyte Activation-1: Eta-1) is a highly phosphorylated and glycosylated phosphoprotein that is expressed in many tissues and has a variety of function, cell adhesion, inhibition of calcium oxalate crystal growth and intracellular signalling. . Osteopontin(OPN) has a cell surface-binding sequence (RGDS), a thrombin cleavage site, many asparatic acid residues, and a number of phosphorylation site. We provide you native form OPN, the monoclonal antibodies and ELISA kit. Human Milk Osteopontin(OPN) is purified from the human first milk by two-step purification procedure as an intact form (50kDa - 80kDa). Anti-OPN monoclonal antibodies were prepared with this native form OPN.

Feature and Advantages

Product : Anti-OPN Monoclonal Antibody
Features : Prepared with native OPN
There are several kinds of clones (See Epitope Analysis)
Use : Detection of native OPN or this fragments with ELISA, western blotting analysis.

Application Examples

Western Blotting analysis with monoclonal antibody from hybridoma clone 8H4. Western Blotting with clone 5D10 Western Blotting with clone 8E5

  Peptide
Clone Name GST/OPN-a GST/OPN-b GST/OPN-c GST/OPN-d GST/OPN-e Purified human milk OPN
2B4 O O O O x O
5D10
(IgG1 K)
O O O O x O
6C3
(note)
O O O x x O
6E5 O O O x O O
7A8 x x x x x O
8B12 ˘ ˘ ˘ ˘ ˘ O
8E5
(IgG1 K)
O O x O x O
8H4 O x x x x O
10G7 x x x x x O
12D4 O O O O x O
17C5 O O x O x O

O : reacts, ˘ : reacts weakly, x : none react, note : 6C3 recognized Thrombin Cleavage Site.

References

1. Alison R Hayman and Timothy M Cox. Tartrate-Resistant Acid Phosphatase Knockout Mice. Journal of Bone and Mineral Research. V.18, No.10, 2003.
2. Andersson G, Ek-Rylander B, Hollberg K, Ljusberg-Sjolander J, Lang P, Norgard M, Wang Y, Zhang SJ. TRACP as an osteopontin phosphatase. J Bone Miner Res. 2003 Oct;18(10):1912-5.
3. Steen Sorensen, Steen Just Justesen, and Anders H. Johnsen. Purification and characterization of osteopontin from human milk. Protein Expression and Purification 30 (2003) 238-245

Product List

Product Name Cat# Quantity Price

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-8H4

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-8E5

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-8B12

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-7A8

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-6E5

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-6C3

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-5D10

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-2B4

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-17C5

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-12D4

50 UL N/A

Anti Osteopontin / OPN, Human (Mouse) Unlabeled DataSheet

HKD-AB02-10G7

50 UL N/A

Product Name Cat# Quantity Price

Anti Osteopontin, Mouse (Rabbit) Unlabeled DataSheet

LSL-LB-4225

100 UL ¥50,000
$667