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Superior to PEG for hybridoma preparation and other cell fusion procedures

GenomONE™ - CF EX Sendai virus (HVJ) Envelope Cell Fusion Kit - News


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Mitochondrial gene replacement in primate offspring and embryonic stem cells.
Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O, Mitalipov S. (Oregon National Primate Research Center, USA.)
Nature. 2009 Sep 17; 461(7262):367-372.
Epub 2009 Aug 26 [PubMed ID: 19710649]

Karyoplasts fusion was carried out by using "SeV (Sendai virus) extract"
which is equivalent to Hemagglutinating virus of Japan (HVJ) envelope (HVJ Envelope: HVJ-E)
with inactivated replication-defective Sendai virus (SeV) particle.

Pronuclear transfer in human embryos to prevent transmission of mitochondrial DNA disease.
Craven L1, Tuppen HA, Greggains GD, Harbottle SJ, Murphy JL, Cree LM, Murdoch AP, Chinnery PF, Taylor RW, Lightowlers RN, Herbert M, Turnbull DM.
Nature. 2010 May 6;465(7294):82-5. doi: 10.1038/nature08958. Epub 2010 Apr 14.
[PMID: 20393463]

Chromosome transfer in mature oocytes.
Tachibana M1, Sparman M, Mitalipov S.
Nat Protoc. 2010 Jun;5(6):1138-47. doi: 10.1038/nprot.2010.75. Epub 2010 May 27.
[PMID: 20539289]

Towards germline gene therapy of inherited mitochondrial diseases.
Tachibana M1, Amato P, Sparman M, Woodward J, Sanchis DM, Ma H, Gutierrez NM, Tippner-Hedges R, Kang E, Lee HS, Ramsey C, Masterson K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer R, Mitalipov S.
Nature. 2013 Jan 31;493(7434):627-31. doi: 10.1038/nature11647. Epub 2012 Oct 24.
PMID: 23103867


Nuclear genome transfer in human oocytes eliminates mitochondrial DNA variants.
Paull D1, Emmanuele V, Weiss KA, Treff N, Stewart L, Hua H, Zimmer M, Kahler DJ, Goland RS, Noggle SA, Prosser R, Hirano M, Sauer MV, Egli D.
Nature. 2013 Jan 31;493(7434):632-7. doi: 10.1038/nature11800. Epub 2012 Dec 19.
[PMID: 23254936]
 

Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos.
Kang E1, Wu G2, Ma H1, Li Y1, Tippner-Hedges R1, Tachibana M3, Sparman M1, Wolf DP1, Schöler HR2, Mitalipov S1.
Nature. 2014 May 1;509(7498):101-4. doi: 10.1038/nature13134. Epub 2014 Mar 26.
[PMID: 24670652]


Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
Yamada M1, Johannesson B1, Sagi I2, Burnett LC3, Kort DH4, Prosser RW4, Paull D5, Nestor MW5, Freeby M3, Greenberg E3, Goland RS3, Leibel RL3, Solomon SL5, Benvenisty N2, Sauer MV4, Egli D5.
Nature. 2014 Jun 26;510(7506):533-6. doi: 10.1038/nature13287. Epub 2014 Apr 28.
[PMID: 24776804]

GenomONE-CF EX (HVJ Envelope Cell Fusion Kit) has been used in this research.

ilisted by permission of the authorsj


GenomONE (HVJ-Envelope)
Applications
(Cell Fusion)

 

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