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Probe molecules such as antibodies, proteins and nucleic acids can be immobilized

Magnetic Particles for Bioseparation (Magnosphere™ S300/Streptavidin)


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Background

Magnosphere™ MS300/Streptavidin particles are Streptavidin coated magnetic microparticles for bioseparation.  The surfaces of Magnosphere MS300/Streptavidin particles are covered with hydrophilic polymer to achieve both low non-specific binding and no inhibition of enzyme activity. Consequently, Magnosphere MS300/Streptavidin particles can be used for PCR amplification systems or  enzyme immunoassay systems.

Application

 

PCR, quantitative PCR
DNA hybridization,
Capturing biotinylated proteins
Enzyme immunoassay

yExample of use 1z Influence on PCR

Added amount of Magnosphere™ to the PCR cocktail (mg/tube)

Various amount of Magnosphere™ MS300/Streptavidin were added to PCR cocktail with defined amount of 100bp DNA and the DNA were amplified by qPCR. Obtained Threshold Cycle (Ct) were plotted against added bead amount. As a result, Ct values were not affected by addition of Magnosphere™ MS300/Streptavidin through 3mg/tube.

yExample of use 2z

Added amount of biotin labeled DNA (copies)

Added vs. immobilized DNA on the Magnosphere™ MS300/Streptavidin. According to the protocol above, various amount of biotin labeled 100bp DNA were reacted with the Magnosphere™ MS300/Streptavidin. The amount of immobilized DNA were quantitated through a qPCR using bead fraction.

 

Feature and Advantages

 

  • High affinity to biotinylated proteins or nucleotides
  • Uniform particle size
  • Superparamagnetic
  • Rapid magnetic responsiveness
  • Low non-specific binding
  • Free of enzyme activity

 

NOTICE:

This product is for research use only and not intended for the other purposes such as therapeutic or diagnostic use. Refer to or verify all regulatory and safety information, and determine suitability of this product before use. The specifications of the product are subjected to change without notice.

Product List

Product Name Cat# Quantity Price

MagnosphereTM MS300/Streptavidin DataSheet ,

JSR-MSP-S300-SA

2 ML ¥40,000
$534

Product Name Cat# Quantity Price

MagnosphereTM MS300/Carboxyl DataSheet ,

JSR-MSP-S300-CA

4 ML ¥48,000
$640

MagnosphereTM MS300/Low Carboxyl DataSheet ,

JSR-MSP-S300-CAL

4 ML ¥48,000
$640

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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