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  • Anti Human Aurora-A Polyclonal Antibody,Rabbit

Anti Human Aurora-A Polyclonal Antibody,Rabbit


For Research Use Only


Aurora-A(Aurora-A/STK-15/Aik)encoding a centrosome-associated kinase, is amplified and overexpressed in many cancer cell types, and is involved in the induction of centrosome duplication-distribution abnormalities and aneuploidy in mammalian cells. So that, Aurora-A is a potential oncogene. Aurora family have been identified three types (Aurora-A, B, C) which has high homology domain in C terminal region. Aurora family is indispensable kinase that regulate the structure and function of centrosomes and spindle. Aurora-A could be one of targeting molecule of anti-cancer therapy.
This antibody was established from the purified serum of the immunized rabbit with N terminal region of Aurora-A and did not show any cross- reactivity to Aurora-B and Aurora-C. This antibody is useful for western blotting and immunohistochemistry.

Package Size 25µg (100µL/vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Below –20°C until needed.
Purification method This antibody was purified from rabbit serum by affinity chromatography.
Working dilution For western blotting ; 0.5~2 µg/mL


green : Aurora-A
blue : DNA

Sample : HeLa Cell

Preparation of antibodies and instruction
Dr.Saya H. and Dr.Marumoto T. at Department of Tumor Genetics and Biology, Kumamoto University School of medicine.

1. Giet R, Prigent C.:J Cell Sci 1999 Nov;112(Pt21):3591-601
2. Bischoff JR,et al.:EMBO J 1988 Jun 1;17(11):3052-65
3. Zhou H,et al.:Nat Genet 1988 Oct;20(2):189-93
4. Lim Shen Kiat,et al.:J Biol Chem 2002 Mar 22;277(12):10719-10726
5. Goepfert TM,et al.:Cancer Res 2002 Jul 15;62(14):4115-22

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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