Anti SulfFP Polyclonal Antibody


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Background

Heparan sulfate and heparin play key roles in the binding of many growth and differentiation factors, and in signaling by other factors. In view of SulfFP’s ability to modify the sulfation of heparan sulfate outside the cell, SulfFPs have been classified as new members of sulfatase family. The SulFP gene is conserved in nematode, fruit fly and human.
SulfFP1 modifies the interaction between heparin binding protein and the carbohydrate side-chain of heparan sulfatate, and has a key role in regulating FGF and Wnt signaling. Changes SulfFP1 in cancer cells have focused attention on these SulfFP as a targeting molecule. SulfFP2 is related enzyme resembling SulfFP1 in structure and activity.

Anti SulfFP1/sulf-1 Polyclonal Antibody Cat#KAL-KM108

Package Size 50µg(200µL/vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of the rabbit immunized with rat SulfFP1, expressed as a recombinant protein in E. coli, and purified by Protein G affinity chromatography.
Working dilution For Western blotting :1.0µg/ml

Western blotting

Sample :SulfFP1-transfected HEK293 cells supernatants

Preparation of antibodies and instruction: Masu M. Nagamine S. University of Tsukuba Graduate School of Comprehensive Human Sciences

Anti SulfFP2/sulf-2 Polyclonal Antibody Cat#KM109
Package Size 25µg(100µL/vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of the rabbit immunized with N-terminal domain of rat SulfFP2, expressed as a recombinant protein in E. coli , and purified by Protein G affinity chromatography.
Working dilution For Western blotting :1.0µg/ml
For Immnocytochemistry :8.0µg/ml
Anti SulfFP2/sulf-2 Polyclonal Antibody Cat#KM110
Package Size 25µg(100µL/vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of the rabbit immunized with C-terminal domain of rat SulfFP2, expressed as a recombinant protein in E. coli , and purified by Protein G affinity chromatography.
Working dilution For Western blotting :1.0µg/ml
For Immnocytochemistry :7.5µg/ml

Western blotting
Sample : SulfFP2-transfected HEK293 cells supernatants


Immnocytochemistry
Sample : SulfFP2-transfected HEK293 cells Preparation of antibodies and instruction:
Masu M.
Nagamine S.
University of Tsukuba
Graduate School of Comprehensive Human Sciences

Product List

Product Name Cat# Quantity Price

Anti SulfFP1 / sulf-1, Rat (Rabbit) Unlabeled DataSheet

KAL-KM108

50 UG ¥49,000
$654

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“Anti SulfFP Polyclonal Antibody” also belongs to the following categories.