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Useful for the research of CD68, macrophage, allergic diseases and delayed hypersensitivity

Anti Human Macrophage (CD68) Monoclonal Antibody


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Background

Macrophages are present in nearly all tissues and organs of the body. They are differentiated from monocytes derived from the bone marrow. Macrophages and monocytes are phagocytes, acting in both innate immunity and cell-mediated immunity of vertebrate animals. Their function is to phagocytize cellular debris and pathogens , and to stimulate lymphocytes and other immune cells to respond to the pathogen.
Macrophages are able to be identified immnohistochemically by virtue of the presence of monocyte/macrophage-associated antigens such as CD68. Antibodies recognizing CD68 have been used as some of the best reagents to detect macrophages in tissues.
CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family.
This antibody recognizes CD68 antigen. The molecular size of the antigen identified by this antibody was 110 kDa. Immunopecipitated antigen by this antibody was also recognized by the other CD68 antibodies such as KP-1 and PG-M1.
In immunohistochemical assays, this antibody recognizes freshly isolated human blood monocytes and tissue macrophages. This antibody also recognizes macrophages obtained from guinea pigs, pigs, bovine species, and monkeys. Since this antibody strongly labels guinea pig macrophages, this antibody will be suitable to examine such macrophages in experimental guinea pig models.
This antibody will be very useful to research of CD68, macrophage, allergic diseases and delayed hypersensitivity.

Package Size 50μg (200μL/ vial)
Format Buffer Mouse monoclonal antibody 0.25mg/mL
PBS [containing 2% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below –20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. PM-1K
Subclass IgG2b,k
Purification method The spleen cells from BALB/c mouse, immunized with human peritoneal cells from patients with endometriosis incubated for 24 hours, were fused with mouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 10μg/mL

Human spleen (frozen section):Red pulp macrophages are positive


Small Intestine of Guinea Pig (paraffin section): Macrophages in lamina propria are positive.

 

Takeya M., Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan

Distribution of positive reactivities of PM-1K with human monocyte/macropahes
Tissues Cells Tissues Cells
Heart Intramuscular Mφ Lymph nodes Mφ in follicles
Mφ in paracortical areas
Lung Alveolar Mφ Pancreas, Salivary, Thyroid,
Adrenals, Urinary bladder,
Prostate, Trachea
Interstitial Mφ
Stomach, Small and Large intestines Mφin lamia propria
Liver Kupffer cells
Mφ in portal triads
Skin Dermal Mφ
Langerhans cells
Kidney Interstitial Mφ
Uriniferous tubule cells
Blood monocytes Freshly isolated monocytes
Spleen Red pulp Mφ
White pulp Mφ
Blood neutrophils Freshly isolated neutrophils
Thymus Mφ in cortex
Mφ in medulla
Myeloid cell lines THP-1
MonoMac6

Mφ@: macrophage

Product List

Product Name Cat# Quantity Price

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The product "Anti Macrophage Scavenger Receptor A antibody Cat#KAL-KT118-S" doesn't contain the additive agent and is filtered through a 0.22 µm filter.


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“Anti Human Macrophage (CD68) Monoclonal Antibody” also belongs to the following categories.