useful for the research of the diabetic, kidney failure, arterial sclerosis,Alzheimer's disease, rheumatoid arthritis and atopic dermatitis

Anti Pentosidine Monoclonal Antibody



Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE). Recent immunological studies using anti-AGE antibody (6D12) demonstrated the presence of AGE-modified proteins in several human tissues: (1) human lens (nondiabetic and noncataractous), (2) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (3) diabetic retina, (4)@peripheral nerves of diabetic neuropathy, (5) atherosclerotic lesions of arterial walls, (6)β2-microglobulin forming amyloid fibrils in patients with hemodialysis-related amyloidosis, (7) senile plaques of patients with Alzheimer’s disease, (8) the peritoneum of CAPD patients, (9) skin elastin in actinic elastosis, and (10) ceriod/lipofuscin deposits. These results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease processes. This antibody named as 6D12 has been used to demonstrate AGE-modified proteins in these human tissues, indicating potential usefulness of this antibody for histochemical identification and biochemical quantification of AGE-modified proteins.

Pentosidine is one of the Maillard compounds identified by Monnier et al in 1989. It has been proved to cross-link Arginine to Lysine residue and be detected inβ2-microglobulin from patients with hemodialysis- related amyloidosis.

 AGEs Antibody Flyer [PDF]
 AGEs Antibody Flyer@(print file) [PDF]
Package Size 50μg (200μL/ vial)
Mouse monoclonal antibody 0.25 mg/mL
Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below 20°C.
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. PEN-12
Subclass IgG1
Purification method The splenic lymphocytes from BALB/c mouse, immunized with pentosidine-HSA were fused to myeloma P3U1 cells. The cell line (PEN-12) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 5-10μg/mL ; for ELISA: 0.1-1.0μg/mL
Specificity Reaction to pentosidine-HSA was suppressed by free-pentosidine in competitive ELISA.

References for Pentosidine
A. Sell, D.R., et al.(1989) : Structure Elucidation of a Senescence Cross-link from Human Extracellular Matrix. J.Biol.Chem.264 : 21597-21602
B. Miyata T, et al.(1996) : Identification of pentosidine as a native structure for advanced glycation end products in ƒÀ2-microglobulin-containing amyloid fibrils in patients with dialysis-related amyloidosis. Proc.Natl.Acad.Sci.USA 93 : 2353-2358

*These references are the background of Pentosidine , and are not this antibody examples.
*This product was developed in conjunction with Meiji Milk Product Co.,LTD Institute of Health Science

References for AGEs antibody
1. Horiuchi, al.: Immunochemical approach to characterize advanced glycation end products of the Maillard reaction; Evidence for the presence of a common structure. J. Biol. Chem. 266: 7329, 1991.
2. Araki, N. et al.: Immunochemical evidence for the presence of advanced glycation end products in human lens proteins and its positive correlation with aging. J. Biol. Chem. 267: 10211, 1992.
3. Miyata, T. et al.: ƒÀ2-Microglobulin modified with advanced glycation end products is a major component of hemodialysis-associated amyloidosis. J. Clin. Invest. 92: 1243, 1993.
4. Yamada, K et al.: Immunohistochemical study of human advanced glycosylation end-products (AGE) in chronic renal failure. Clin. Nephrol. 42: 354, 1994.
5. Kume, S. et al.: Immunohistochemical and ultrasturactural detection of advanced glycation end products in atherosclerotic lesions of human aorta using a novel specific monoclonal antibody. Am. J. Pathol. 147 : 654, 1995.
6. Makino, H. et al.: Ultrastructure of nonenzymatically grycated mesangial matrix in diabetic nephropathy. Kidney International 48: 517, 1995.
7. Mori, T. et al.: Localization of advanced grycation end products of Maillard reaction in bovine tissues and their endocytosis by macrophage scavenger receptors. Exp. Molec. Pathol. 63:135, 1995
8. Miyata, T. et al.: Identification of pentosidine as a native structure for advanced glycation end products inƒÀ2-Microglobulin forming amyloid fibrils in patients with dialysis-related amyloidsis. Proc. Natl. Acad. Sci. USA. 93: 2353, 1996
9. Kimura, T. et al.: Accumulation of advanced glycation end products of the Maillard reaction with age in human hippocampal neurons. Neurosci. Lett. 208: 53,1996.
10. Ikeda, K. et al.: NƒÃ-(carboxymethyl) lysine protein adduct is a major immunological epitope in proteins modified with advanced glycation end products of the Maillard reaction. Biochemistry 35: 8075,1996.


Note : These products cannot be sold to USA because of the patent issue.

Product List

Product Name Cat# Quantity Price

Anti Pentosidine, - (Mouse) Unlabeled DataSheet


50 UG ¥55,000

Anti Pentosidine, - (Mouse) Biotin DataSheet


50 UG ¥70,000

Anti Pentosidine, - (Mouse) Horseradish Peroxidase DataSheet


50 UG ¥70,000

Note : Product "KAL-KH012""KAL-KH012-01""KAL-KH012-02" cannot be sold to USA due to patent issues.

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