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specific to rat macrophages and dendritic cell populations in various organs and tissues

Anti Rat Macrophage/Dendritic Cells Monoclonal Antibody


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Background

This anti-rat macrophage/dendritic cells monoclonal antibody, RM-4, was produced by using a homogenate of silica-induced lung granulomas of rat as immunogen. Immunohistochemistry demonstrated that RM-4 is specific for rat macrophages and dendritic cell populations in various organs and tissues.
In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigen, of which molecular weight is 46kDa, presenting on the membrane of endosomes and lysosomes in macrophages and dendrictic cells.
Thus, this antibody is very useful for not only in discriminating macrophage/dendritic cells from various cell populations but also in identifying endosomes and lysosomes.


Rat liver (frozen section) Kupffer cells are positively staine

Rat spleen (frozen section)) Macrophages in red pulp and white pulp were positivery stained
Takeya M., Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto, Japan

Specificity

Organs/Tissues Positive Cells
Spleen Red pulp macrophages
White pulp
  TBMs
  IDCs in PALS
  Marginal zone macrophages
  Marginal metallophils
Lymph nodes TBMs in follicles
IDCs in paracortical areas
Sinus macrophages
Thymus Macrophages in cortex and medulla
IDCs in medulla
Lungs Alveolar macrophages
Interstitial macrophages
Omentum Rounded macrophages
Twisted spindle shaped macrophages
Peyer’s patches Macrophages in the lymphoid follicle
Dendritic cells in the interfollicular area
Skin LCs
Dermal macrophages
Brain Microglial cells in the parenchyma
Macrophages in the subarachnoid spaces
Macrophages in the perivascular space
Connective tissue Macrophages
Peritoneal cavity Peritoneal macrophages

TBM=tingible body macrophages; IDCs=interdigitating cells;
PALS=periarteriolar lymphatic sheath; LCs=Langerhans cells.

<References>
1. Iyonaga K., Takeya M., Yamamoto T., Ando M., Takahashi K. (1997) A novel monoclonal antibody, RM-4, specifically recognizes rat macrophages and dendritic cells in formalin-fixed, paraffin-embedded tissues. Histochem J. 29:105-116

Package Size 50μg (200μL / viaL)
Format Mouse monoclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below –20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided
Clone No. RM-4
Subclass IgG1
Purification method The spleen cells from BALB/c mouse, immunized with homogenate of silica-induced lung granuloma of rat, were fused to myeloma NS-1 cells. The screening of the hybridoma cells was performed on cryostat sections of rat spleens. The hybridoma cell line (RM-4) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 10μg/mL on frozen sections and paraffin sections.
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KAL-KT014

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