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useful for not only in discriminating monocyte / macrophages from various cell populations but also in identifying lysosomes and their related structures in macrophages

Anti Rabbit Macrophage / Monocyte Monoclonal Antibody


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Background

This anti-rabbit macrophage monoclonal antibody, RbM2, was produced by using thiogllycate-elicited rabbit peritoneal macrophages as immunogen.  Immunoelectoron microscopy demonstrates that RbM2 reacts with lysosomes of rabbit macrophages and monocytes. This selective reactivity was confirmed in various experiments by endocytosis. In contrast, dendritic cells, such as follicular dendritic cells (FDCs) of lymphoid follicles, interdigitating cells(IDCs) of lymphoid T zone, or epidermal Langerhan’s cells, are not reactive with this antibody.
The antigen recognized by RbM2 is a lysosomal membrane protein with 50,000 molecular weight.
Thus, this antibody is very useful for not only in discriminating monocyte / macrophages from various cell populations but also in identifying lysosomes and their related structures in macrophages.


Rabbit Liver (frozen section): Kupffer cells are positively stained.
Takeya M., Second Department of Pathology,
Kumamoto University School of Medicine, Kumamoto, Japan

Specificity

Organ Reaction
Positive negative
Thymus Macrophages in cortex
Macrophages in medulla
Epithelial cells
Dendritic cells
Spleen Red pulp macrophages IDCs in PALS
Marginal zone macrophages
FDCs in the germinal centre
Lymph nodes TB macrophages in follicles
Macrophagesim sinuses macrophage
IDCs in paracortical areas
FDCs in the germinal center
Lungs Alveolar macrophages  
Liver Kupffer cells Sinusodal endothelia
Perisinusoidal fat-storing cells
Parenchymal cells
Skin Dermal macrophages Langerhans cells
Epidermal dendritic cells
Brain Macrophages in the subarachnoid space Microglial cells
others Monocytes
Peritoneal macrophages
Macrophages in the milky spot of the omentum
Granulocytes
Lymphocytes
Erythrocytes
Polymorphonuclear cells
Neuronal cells
Muscle cells

PALS=periarteriolar lymphatic sheath
TB=tingible body
IDCs=interdigitating cells
FDCs=follicular dendritic cells

<References>
1. Shimokawa Y., Takeya M., Miyauchi Y., Takahashi K. (1990): A monoclonal antibody, RbM2, specific for a lysosomal membrane antigen of rabbit monocyte/macrophages. Immunol. 70: 513-519
2. Ruan Y., Takahashi K., Naito M. (1995): Immunohistochemical detection of macrophage-derived foam cells and macrophage clony-stimulating factor in pulmonary atherogenesis of cholesterol-fed rabbit. Pathol Int. 45(3): 185-195
3. Yoshimura N., Arima S., Nakayama M., Sato T.,  Takahashi K.(1994): Renal impairment and intraglomerular mononuclear phagocytes in choresterol-fed rabbits. Nephron. 68(4): 473-480

Package Size 50μg (200μL / vial)
Format Mouse monoclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Store below –20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided
Clone No. RbM2
Subclass IgG1
Purification method The spleen cells from BALB/c mouse, immunized with thioglycate-elicited rabbit peritoneal macrophages, were fused to myeloma NS-1 cells. The screening of the hybridoma cells was performed on cryostat sections of rabbit spleens. The hybridoma cell line (RbM2) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.
Working dilution for immunohistochemistry: 10μg/mL on frozen sections, not applicable for paraffin sections.
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