Anti Ccd1 Polyclonal Antibody


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Background

The novel zebrafish protein Ccd1 (Coiled-coil-DIX1) possesses a C-terminal DIX (Dishevelled-Axin) domain as well as an N-terminal coiled-coil domain. The DIX domain proteins Ccd1, Axin, and dishevelled (Dvl / Dsh) are important in Wnt signaling. Ccd1 forms a heteromeric complex with Axin and Dvl/Dsh and regulates neural patterning through Wnt pathway activation. This antibody presented here reacts with the coiled-coil domain of the Ccd1 isoforms Ccd1A, Ccd1B, Ccd1C.
iBrain Res Mol Brain Res., 2005 Apr 27; 135(1-2):169-80j.


Immnocytochemistry
Sample:
AFZebrafish Ccd1-trans‚†ected Hela cells
BFMouse Ccd1B-trans‚†ected Hela cells

Preparation of antibodies and instruction:
Masu M.@Shiomi K.
University of Tsukuba
Graduate School of Comprehensive Human Sciences

Package Size 25µg (100µL/vial)
Format Rabbit polyclonal antibody (0.25mg/mL)
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteriostat]
Storage Store below |20®C
Once thawed, store at 4®C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of a rabbit immunized with a peptide fragment of Ccd1.
Purified by peptide affinity chromatography.
Working dilution For Western blotting : 1.0µg/ml
For Immnocytochemistry : 1.0`2.0µg/ml
Product List

Product Name Cat# Quantity Price

Anti Ccd1, Zebrafish (Rabbit) Unlabeled DataSheet

KAL-KM107

25 UG ¥49,000
$654

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