useful for the research of MCM3/GANP complex phosphorylation state during cell cycle progression

Anti G5PR polyclonal antibody


print

Background

G5PR was cloned as novel protein which could bind to Germinal center Associated DNA PrimaseiGANPj. GANP is involved in important events that regulate DNA replication in activated germinal centre B cells. G5PR is homologous to known regulatory B’’ subunits of PP2A, and the G5PR protein is associated with PP5 and PP2A, suggesting that G5PR is a GANP-associated PPase component. G5PR contributes to the regulation of the phosphorylation state of the chromatin-bound or nonbound form of MCM3 (minichromosome replication molecule 3) in vivo. So that, G5PR might regulate MCM3/GANP complex phosphorylation state during cell cycle progression.
This antibody is very useful for immunoprecipitation and westernblotting.

Application

Working dilution for immunoprecipitation : 2 μg
Working dilution for western blotting : 1`5 μg/mL

Westernblotting
Sample : Myc-GFP-G5PR co-transfected cell lycate
? Immunoprecipitated by anti-G5PR antibody, then ditected by anti-Myc antibody
?Immunoprecipitated by anti-Myc antibody, then ditected by anti-G5PR antibody

Preparation of antibodies and instruction
Drs.Sakaguchi N. and Igarashi H. at Department Immunology, Kumamoto University School of Medicine

Character

Package Size 25μg (100μL / vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer Block Ace as a stabilizer, containing 0.1% Proclin as a bacteriostat
Storage Below -20°C
Purification method This antibody was purified from rabbit serum by affinity chromatography.

<Reference>
1. Genes Cells 2002 Aug;7(8):821-34

Product List

Product Name Cat# Quantity Price

Anti G5PR, Mouse (Rabbit) Unlabeled DataSheet

KAL-KR058

25 UG ¥49,000
$654

Product Name Cat# Quantity Price

Anti Aurora A, Human (Rabbit) Unlabeled DataSheet

KAL-KR051

25 UG ¥49,000
$654

Anti SIAH-1, Human (Rabbit) Unlabeled DataSheet

KAL-KR055

25 UG N/A

Anti SIAH 2, Human (Rabbit) Unlabeled DataSheet

KAL-KR056

25 UG ¥49,000
$654

Anti G5PR, Mouse (Rabbit) Unlabeled DataSheet

KAL-KR058

25 UG ¥49,000
$654

Anti Ajuba, Human (Rabbit) Unlabeled DataSheet

KAL-KR068

25 UG ¥49,000
$654

Anti MDC1 / NFBD1 / KIAA0170, Human (Rabbit) Unlabeled DataSheet

KAL-KR087

25 UG ¥49,000
$654

Anti Phospholipid Scrambrase 2, Mouse (Rabbit) Unlabeled DataSheet

KAL-KR097

25 UG ¥49,000
$654

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



“Anti G5PR polyclonal antibody” also belongs to the following categories.