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  • Anti Rat Oazin (Antizyme inhibitor 1) Monoclonal Antibody (Clone No. HI-12)

useful for the research in polyamine biosynthesis pathway.

Anti Rat Oazin (Antizyme inhibitor 1) Monoclonal Antibody (Clone No. HI-12)


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Background

ODC (ornithine decarboxylase) is a key enzyme in polyamine biosynthesis pathway. The degradation of ODC catalyzed by the 26S proteasome is accelerated by antizyme, an ODC inhibitory protein induced poliamine. Antizyme inhibitor 1 (AZI) specifically binds to antizyme with a higher affinity than that of ODC and thus can release active ODC from inactive ODC-antizyme complex. AZI consists of about 450 amino acids and 50 kDa protein. AZI is highly homologous to ODC but retains no enzymatic activity. AZI is a short-living protein that undergoes proteasomal degradation and the degradation is inhibited by interaction with antizyme.
AZI is induced in cells and tissues following growth stimulation, is elevated in some tumors. AZI overexpression leads to increased rates of cell proliferation in the rat carcinoma cells and mouse fibroblasts and this effect appears to be partially independent of the ability of AZI to interact with antizyme. It has been also reported that antizyme and AZI localize to centrosome and may play an important role in the regulation of centrosome homeostasis and oncogenesis.

Application

WB 0.25 μg/mL
IHC Not tested
ICC 30 μg/mL
ELISA Not tested
FCM Not tested
Neutralization Not tested
IP Not tested
Western blot detection of antizyme inhibitor with the monoclonal antibody.
Sample: extract of HTC (rat hepatoma) cells (20 μg).
Separation: 11% SDS-PAGE.
Membrane: Hybond-P.
Primary antibody: HI-12 monoclonal antibody at 260 ng/ml.
Secondary antibody: horseradish peroxidase-conjugated anti-mouse or rat IgG (Histofine Simple Stain MAX-PO, Nichirei Bioscience) at a dilution of 1:200,000.
(Data from Dr. Y. Murakami)
Fluorescent immunocytochemistry of antizyme inhibitor.
Cell: HTC cells.
Primary antibody: HI-12 monoclonal antibody at 40 μg/ml.
Secondary antibody: anti-mouse immunoglobulin conjugated with Alexa 546 (Invitrogen) (red).
The nuclei were stained with TO-PRO 3 (Invitrogen) (blue).
(Data from Dr. Y. Murakami)

Character

Primary Source  
Type Monoclonal
Immunogen affinity-purified Antizyme inhibitor from rat liver
Raised in Mouse
Myeloma P3-NSI
Clone number HI-12
Isotype IgG3κ
Source Ascites
Purification notes DEAE
Cross Reactivity Human,Mouse
Concentration 0.25 mg/mL
Contents(Volume) 50 μg(200 μL/vial)
Label Unlabeled
Buffer PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]
Storage Store below –20 &degC. Once thawed, store at 4 &degC. Repeated freeze-thaw cycles should be avoided.

<Reference>
1. Murakami Y. et al.: Properties and fluctuations in vivo of rat liver antizyme inhibitor. Biochem J. 1989 May 1;259(3):839-45.
2. Murakami Y. et al.: Cloning of antizyme inhibitor, a highly homologous protein to ornithine decarboxylase. J Biol Chem. 1996 Feb 16;271(7):3340-2.
3. Nakatani T. et al.: Restricted Zn2+ availability affects the antizyme-dependent ornithine decarboxylase degradation pathway inisolated primary cultured rat hepatocytes. Biochem Biophys Res Commun. 1998 Feb 24;243(3):797-800.
4. Kim SW. et al.: Regulation of cell proliferation by the antizyme inhibitor: evidence for an antizyme-independent mechanism. J Cell Sci. 2006 Jun 15;119(Pt 12):2583-91. Epub 2006 May 30
5. Mangold U. et al.: Antizyme, a mediator of ubiquitin-independent proteasomal degradation and its inhibitor localize to centrosomes andmodulate centriole amplification. Oncogene. 2008 Jan 24;27(5):604-13. Epub 2007 Jul 30.

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



“Anti Rat Oazin (Antizyme inhibitor 1) Monoclonal Antibody (Clone No. HI-12)” also belongs to the following categories.