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Anti Calnexin Ģ185-520 Monoclonal Antibody (Clone.No.2E9)


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Background

This antibody doesn't react to Calnexin, and recognizes only Calnexin Δ185-520.


Preparation of antibodies and instruction F
Kai H
Department of Molecular Medicine,
Faculty of Medical and Pharmaceutical Sciences,@Kumamoto University

<Reference>
1. Okiyoneda T, Wada I, Jono H, Shuto T, Yoshitake K, Nakano N, Nagayama S, Harada K, Isohama Y, Miyata T, Kai H.@ Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator. FEBS Lett. 2002 Aug 28;526(1-3):87-92.
2.Okiyoneda T, Kai H. Introduction of the conventional method of DNA transfection by adenovirus vector Nippon Yakurigaku Zasshi. 2002 Jun;119(6):337-44. Japanese.

Character

Package Size 50µgi200µL/vialj
Format Mouse Monoclonal Antibody 0.25mg/mL
Buffer PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. 2E9
Sub Class IgG1
Purification method The splenic lymphocytes from BALB/c mouse, immunized with partial peptide of Calnexin Δ185-520 were fused to myeloma P3U1 cells. The hybridoma is proliferated by serum free medium, and collects the conditioned medium, and IgG is refined with Protein G Affinity column.
Working dilution For western blotting ;0.2`1.0µg/mL
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