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Anti Ca2+ /Calmodulin-Dependent Protein Kinase II d1-d4 Polyclonal Antibody


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Background

Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) may play key roles in various Ca2+ -induced cellular functions. Interestingly, many kinds of isoforms have been identified in various tissues or cells. Recently, it has been revealed that some isoforms are located in specific regions in the cells, so it is speculated that these isoforms have physiologically particular functions in each region.
There are four different isoforms such as α,β,γandδ. This antibody reacts with δ1]δ4 splice variants. Immunochemical studies indicate that δ2 is expressed in various tissues or cells such as insulinoma cells and that δ3 is abundant in the nucleus in cerebellar granule cells. These results suggest thatδ3 is involved in Ca2+ -dependent gene expression.
This antibody has been proved to be useful for the immunoblotting and immunohistochemistry

Application

Immunohistochemistry : 15μg/mL
Immunoblotting : 10`20μg/mL

Immunoblotting

Sample:
(1)@MIN6 (control)
(2)@MIN6 (after overexpression of δ‚Q)

Preparation of antibodies and instruction
Hideyuki Yamamoto
Department of Pharmacology and Neuropsychiatry, Faculty of Medicine, Kumammoto University, Japan

Character

Package Size 200μg ( 200μL / vial )
Format Rabbit polyclonal antibody 1 mg/mL
Buffer 0.1% Proclin as bacteriostat, 50% Block Ace as a stabilizer in PBS
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was purified from rabbit serum immunized with 15-amino acid segment from unique carboxyl-terminal ends of CaM kinaseII δ1-δ4 isoforms by peptide affinity chromatography.
Working dilution Immunohistochemistry: 15μg/mL ,for immunoblotting : 10`20μg/mL  

<Reference>
1. Matumono,K., Ebihara,K., Yamamono, H., Tabuchi, H., Fukunaga, K., Yasunami, M., Ohkubo, H., Shichiri, M.,Miyamoto, E: J.Biol.Chem.,274,2053-2059 (1999)
2. Tabuchi, H., Yamamoto, H., Matsumoto, K., Ebihara, K., Takeuchi, Y., Fukunaga,K.,Hiraoka,H., Sakai Y., Shichiri,M., Miyamoto,E. :Endocrinology,141,2350-2360 (2000)
3. Takeuchi, Y., Yamamoto. H., Matsimoto, K., Kimura, T., Katsuragi, S., Miyakawa, T., Miyamoto, E.: J.Neurochem.,72.815-825 (1999)
4. Takeuchi, Y., Yamamoto, H., Miyakawa, T., Miyamoto, E.: J. Neurochem.,74,1913-1922 (2000)
5. Takeuchi, Y., Yamamoto, H., Fukunaga, K., Miyakawa, T., Miyamoto, E. : J. Neurochem.,74,2557-2567 (2000)

Product List

Product Name Cat# Quantity Price

Anti CaM Kinase II Δ1/2/3/4, Rat (Rabbit) Unlabeled DataSheet

KAL-KY041

200 UG ¥49,000
$654

Product Name Cat# Quantity Price

Anti CaM Kinase II Δ1/2/3/4, Rat (Rabbit) Unlabeled DataSheet

KAL-KY041

200 UG ¥49,000
$654

Anti Aldehyde Oxidase, Rat (Rabbit) Unlabeled DataSheet

KAL-KR063

25 UG ¥49,000
$654

Anti Adenosine Deaminase2, Chicken (Rabbit) Unlabeled DataSheet

KAL-KR081

25 UG ¥49,000
$654

Anti Indoleamine-2,3 dioxygenase / IDO, Mouse (Rabbit) Unlabeled DataSheet

KAL-KR101

150 UG ¥49,000
$654

Anti ATP1B3, Human (Mouse) Unlabeled DataSheet

KAL-KB465

50 UG N/A

Anti NEDD4L, Human (Mouse) Unlabeled DataSheet

KAL-KB521

50 UG N/A

Anti NUDT9, Human (Mouse) Unlabeled DataSheet

KAL-KB523

50 UG N/A

Anti ATP2C1, Human (Mouse) Unlabeled DataSheet

KAL-KB542

50 UG N/A

Anti PDCD8, Human (Mouse) Unlabeled DataSheet

KAL-KB543

50 UG N/A

Anti DDX11, Human (Mouse) Unlabeled DataSheet

KAL-KB545

50 UG N/A

Anti CYB5R3, Human (Mouse) Unlabeled DataSheet

KAL-KB547

50 UG N/A

Anti UQCRC2, Human (Mouse) Unlabeled DataSheet

KAL-KB556

50 UG N/A

Anti UQCRC1, Human (Mouse) Unlabeled DataSheet

KAL-KB557

50 UG N/A

Anti EME1, Human (Mouse) Unlabeled DataSheet

KAL-KB559

50 UG N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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