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Anti Human CD298(Na/K-ATPase b3) Monoclonal Antibody


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Background

Na/K-ATPase is a membrane-associated enzyme responsible for the active transport of Na + and K+, and the maintenance of Na+ and K+ gradients across membrane. It is a heterodimer of two different kinds subunit, a large α subunit and a smaller glycoprotein subunit β. At least four distinct α subunit and four β subunit isoforms have been identified. In some tissues, it has been showed that Na/K-ATPase is associated with third subunit γ, a small regulatory protein.
CD298 (also known as Na/K-ATPase β3) is a 42-kDa glycoprotein and one of β subunit isoforms. CD298 has been detected in amphibians, mouse, rat, and human. In rat, CD298 expresses in a variety of tissues including testis, brain, kidney, spleen, stomach, small intestine, colon, lung, and liver. In the retina Na/K-ATPase restores Na+@and K+ gradients used by photoreceptor dark current, synaptic activity, action potential, and CD298 is largely restricted to photoreceptors in the mouse and rat. In human, CD298 also involves in regulation of T and B lymphocyte proliferation. This antibody is specific to human CD298 and will be useful for FCM, immunoprecipitation, immunohistochemistry.

Application

WB Not tested
IHC 5.0-10.0 μg/mL
ICC Not tested
ELISA Not tested
FCM 0.5-1.0 μg/mL
Neutralization Not tested
IP 5.0-10.0 μg/mL

Character

Primary Source HGNC:806
Type Monoclonal
Immunogen Human Cancer Cell Line
Raised in Mouse
Myeloma P3U1
Clone number 5G10
Isotype IgG2a,
Source Serum Free Medium
Purification notes ProteinG
Cross Reactivity Not yet tested in other species.
Concentration 0.25 mg/mL
Contents(Volume) 50 μg(200 μL/vial)
Label Unlabeled
Buffer PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]
Storage Store below –20 °C. Once thawed, store at 4 °C. Repeated freeze-thaw cycles should be avoided.

<Reference>
1. Malik N. et al.: Identification of the mammalian Na,K-ATPase beta3 subunit. J Biol Chem.1996 Sep 13;271(37):22754-8.
2. Arystarkhova E. et al.: Tissue-specific expression of the Na,K-ATPase beta3 subunit. The presence of beta3 in lung and liver addresses the problem of the missing subunit. J Biol Chem.1997 Sep 5;272(36):22405-8.
3. Blanco G. et al.: Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function. Am J Physiol.1998 Nov;275(5 Pt 2):F633-50. Review.
4. Wetzel RK. et al.: Cellular and subcellular specification of Na,K-ATPase alpha and beta isoforms in the postnatal development of mouse retina. J Neurosci. 1999 Nov 15;19(22):9878-89.
5. Chiampanichayakul S. et al.: Engagement of Na,K-ATPase beta3 subunit by a specific mAb suppresses T and B lymphocyte activation.
Int Immunol.2002 Dec;14(12):1407-14.

Product List

Product Name Cat# Quantity Price

Anti CD298, Human (Mouse) Unlabeled DataSheet

KAL-KS131

50 UG ¥55,000
$734

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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