Medaka Vitellogenin (Vg) ELISA Kit


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Background

In recent years the influence of Endocrin-Disrupting-Chemicals (EDC) upon living organisms has been one of the hot topics in environmental study. However, determination of the chemical compound species that have EDC-like influence has not yet been completed, and it is absolutely necessary to take actions to complete such a task.
Vitellogenin (Vg), a precursor of yolk protein, is a female-specific protein that is expressed only in the blood of higher vertebrate oviparous organisms. Vg is normally expressed in liver under regulation of estrogen (female sex hormome) during egg yolk formation process, and when it reaches ovum via blood stream it contributes to the egg yolk formation. Since estrogen has been known to have an effect upon male species to induce unusual expression of Vg, an ELISA screening method detecting the Vg in EDC-exposed samples has become the most attractive bioscreening assay system.

Application

<Standard curve>

<Reproducibilities>

Inter assay(n=20)
Sample A B
Mean (ng/mL) 30.1 14
SD 1.4 0.4
CV (%) 4.7 3.1
Intra assay@(n=20)
Sample A B
Mean (ng/mL) 32.5 14.8
SD 1.9 1.2
CV (%) 6 8.4
Specificity: cross reactivity

Medaka  Carp  Mummichog
Immunoblotting (+) (-) (+)

Kit component

(1) Antibody-coated microtiter plate (8 wells×12) 1 plate
(2) The standard, Medaka Vg, lyophilized 200μl × 2
(3) Dilution solution 30mL × 2
(4) Biotin conjugated anti-medaka Vg antibody (×100) 80μl × 1
(5) HRP-conjugated streptavidin (×500) 20μl × 1
(6) OPD (o-phenylendiamin) tablets 2 tab.
(7) Substrate buffer 15 mL × 1
(8) Stop solution 6 mL@ × 1
(9) Wash solution (×20) 30 mL  × 1

Principle

This kit is based on the ELISA method which is used with specific anti- medaka polyclonal antibody.
Anti- medaka polyclonal antibody is coated on the microplate, so that Vg in the sample and standard is captured. After reacting biotin-conjugated anti-medaka polyclonal antibody and HRP-conjugated streptavidin, the complex of coated-antibody|antigen|antibody is formed. Finally, you could measure the Vg concentration depend on the coloring strength which has developed with enzymatic reaction.

Product List

Product Name Cat# Quantity Price

Vitellogenin, ELISA, Killifish DataSheet

KAL-KH075

1 KIT N/A

Product Name Cat# Quantity Price

Vitellogenin ELISA Carp DataSheet

KAL-KH003

1 KIT N/A

Vitellogenin ELISA, Quail DataSheet

KAL-KH049

1 KIT N/A

Product Name Cat# Quantity Price

Anti Vitellogenin, Killifish (Mouse) Unlabeled DataSheet

KAL-KH006

100 UG ¥40,000
$534

Anti Vitellogenin : F10-2, Killifish (Mouse) Unlabeled DataSheet

EBT-AVMF10-2-EX

1 ML ¥50,000
$667

Anti Vitellogenin : D3-3, Killifish (Mouse) Unlabeled DataSheet

EBT-AVMD3-3-EX

1 ML ¥50,000
$667

Anti Vitellogenin, Killifish (Mouse) Horseradish Peroxidase DataSheet

EBT-MVD33-HRP-EX

0.5 ML ¥50,000
$667

Anti Vitellogenin, Fish (Mouse) Unlabeled DataSheet

KAL-KH005

100 UG ¥40,000
$534

Anti Vitellogenin : B6-1, - (Mouse) Unlabeled DataSheet

EBT-MUMB6-1-EX

1 ML N/A

Anti Vitellogenin : F61-2, - (Mouse) Unlabeled DataSheet

EBT-MUMF61-2-EX

1 ML N/A

Anti Vitellogenin : ZV2-1, Zebrafish (Mouse) Unlabeled DataSheet

EBT-ZV2-1-EX

1 ML N/A

Anti Vitellogenin : Z42-7, Zebrafish (Mouse) Unlabeled DataSheet

EBT-Z42-7-EX

1 ML N/A

Anti Vitellogenin, Zebrafish (Mouse) Horseradish Peroxidase DataSheet

EBT-ZV21-HRP-EX

0.5 ML N/A

Anti Vitellogenin Carp  (Mouse) Unlabeled DataSheet

KAL-KH004

100 UG ¥40,000
$534

Anti Vitellogenin, - (Mouse) Unlabeled DataSheet

EBT-FHM1271-EX

1 ML N/A

Anti Vitellogenin, - (Mouse) Unlabeled DataSheet

EBT-FHM1671-EX

1 ML N/A

Anti Vitellogenin, - (Mouse) Unlabeled DataSheet

EBT-FHM883-EX

1 ML N/A

Anti Vitellogenin, Fish (Mouse) Unlabeled DataSheet

KAL-KH007

100 UG ¥40,000
$534

Product Name Cat# Quantity Price

Anti Metallothionein, Rabbit (Mouse) Unlabeled DataSheet

KAL-KA009

100 UG ¥45,000
$600

Anti Metallothionein, Rabbit (Mouse) Biotin DataSheet

KAL-KA009-01

100 UG ¥70,000
$934

Anti Estrogen Receptor α, Alligator (Rabbit) Unlabeled DataSheet

KAL-KR092

25 UG ¥49,000
$654

“Medaka Vitellogenin (Vg) ELISA Kit” also belongs to the following categories.