Carp Vitellogenin (Vg) ELISA Kit


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Background

Vitellogenin (Vg) is a precurser of egg york and detected female serum near ovulation. Vg is not detected in male serum, but treated with 17β-estradiol, Vg is detectable in male serum. Carp Vg ELISA kit is constructed as a sandwich ELISA format using anti carp Vg antibodies and titrate serum carp Vg.

Application

<Standard curve>
The standerd curve of carp Vg is obteined by measurement of the serial dilution of standerd carp Vg solution with the kit, as is shown in the figure

 

<Sensitivity>
Dynamic range:@7.8-500 ng/mL

<Reproducibilities>

Inter assay(n=14)
Sample 1 2 3
Mean (ng/mL) 219.3 178 100
SD 7.5 13.1 0.4
CV(%) 3.4 7.4 4.2
Intra assay@(n=9)
Sample 1 2 3
Mean (ng/mL) 250.6 195 118
SD 21 17.4 11
CV(%) 8.4 8.9 9.1

<Recovery of exogenous vitellogenin >
Plasma Av. 95.6%

Kit component

(1) Antibody-coated microtiter plate (8wells×12) 1 plate
(2) The standard, Carp Vg, lyophilized 2 vials
(3) Dilution solution 30 mL
(4) HRP-conjugated anti Carp Vg antibody (×500) 30 μL
(5) Substrate buffer 15 mL
(6) OPD (o-phenylendiamin) tablets 2 tab.
(7) Wash solution (×20) 30 mL
(8) Stop solution 6 mL

Principle

This kit is constructed using the antibodies specific to carp Vg , as both capture and secondary antibodies of sandwich ELISA format. During the assay, carp Vg in the samples is captured by the antibody, immobilized on the surface of microplate wells, and simultaneously reacts with the horseradish peroxidase (HRP) labeled antibody. Quantitation of carp Vg in samples can be made by measurement of HRP activity of the complex, which is exponential to the quantity of carp Vg.

Product List

Product Name Cat# Quantity Price

Vitellogenin ELISA Carp DataSheet

KAL-KH003

1 KIT N/A

Product Name Cat# Quantity Price

Vitellogenin ELISA Carp DataSheet

KAL-KH003

1 KIT N/A

Vitellogenin ELISA, Quail DataSheet

KAL-KH049

1 KIT N/A

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1 ML N/A

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1 ML N/A

Anti Vitellogenin, Zebrafish (Mouse) Horseradish Peroxidase DataSheet

EBT-ZV21-HRP-EX

0.5 ML N/A

Anti Vitellogenin Carp  (Mouse) Unlabeled DataSheet

KAL-KH004

100 UG ¥40,000
$534

Anti Vitellogenin, - (Mouse) Unlabeled DataSheet

EBT-FHM1271-EX

1 ML N/A

Anti Vitellogenin, - (Mouse) Unlabeled DataSheet

EBT-FHM1671-EX

1 ML N/A

Anti Vitellogenin, - (Mouse) Unlabeled DataSheet

EBT-FHM883-EX

1 ML N/A

Anti Vitellogenin, Fish (Mouse) Unlabeled DataSheet

KAL-KH007

100 UG ¥40,000
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KAL-KR092

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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