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Anti Red Sea Bream Vitellogenin Monoclonal Antibody [Clone No. 5A4]


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Background

&Vitellogenin (Vg) is a precurser of egg york and detected female serum near ovulation. Vg is usually synthesized by effect of estrogen (female hormone) in the female liver in the vitellogenic stage, is taken in the egg through the blood inside and composes the yolk protein.
Vg is not detected in male serum, but treated with 17β-estradiol, Vg is detectable in male serum. Recently, Vg is paid to attention as a biomarker of the environmental endocrine disruptor that exists in environmental water such as rivers.

Application

<cross reactivity>
  Red sea bream Carp Mummichog Medaka
ELISA (+) (|) (|) (|)
Immunoblotting (+) (+) (|) (|)

<ELISA>
Antigen :
vitellogenins were purified from male fish induced with 17βestradiol and carp vitellogenin was purified from female. .Each antigen were diluted to 1μg/mL and added to microtiter plate.
Primary antibody : Purified monoclonal antibody(5A4)
Working dilution : ELISA 0.25`2μg/ml(Fixed quantity system by ELISA has not been established.)

< IMMUNOBLOTTING>
Sample :
Plasma(dilution 1:40) from female fish (carp) or male fish induced with 17β- estradiol (red sea bream, mummichog and medaka).
Primary antibody :
5A4 diluted 1:1000
Working dilutionFImmunoblotting 0.1`1μg/ml

Character

Package Size 100μg(100μl/vial)
Format Mouse monoclonal antibody 1.0mg/ml
Buffer 10mM sodium phosphate , 0.15M sodium chloride , 1%BSA , pH7.4
Storage Store below –20°C.
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Clone No. 5A4
Subclass IgG1
Immunogen souce Red sea bream vitellogenin purified from plasma of 17β-estradiol induced fish

<Reference>
1).N.Hiramastu, M.Shimizu, H.Fukada, M.Kitamura, K.Ura, H.Fuda, and A.Hara, (1997) Comp. Biochem Physiol. 118C(2) 149-157 
2).N.Hiramatsu and  A.Hara. (1996) Comp Biochem Physiol. 115A(3) 243-251 
3).H.Okumura, A.Hara, F.Saeki, T.Todo, S.Adachi and K.Yamauchi,(1995) Fisheries Science , 61(2) 283-289
4).T.Matsubara, T.Wada and A.Hara, (1994) Comp Biochem Physiol. 109B(4) 545-555@
5).Y.Tao, A,Hara, R.. G. Hodoson, L. C. Woods lll and C. V. Sullivan, (1993) Fish Physiol. Biochem, 12(1) 31-46
6) A.Hara, C. V. Sullivan and W.W. Dickhoff,(1993) Zool . Sci., 10(2), 245-256

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“Anti Red Sea Bream Vitellogenin Monoclonal Antibody [Clone No. 5A4]” also belongs to the following categories.