one step culture cell storage solution.

COS Banker Instructions


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Background

Cos Banker is a one step culture cell storage solution. Suspend culture cells in Cos Banker and store at –80 degrees for long term storage (it is not necessary to store in liquid nitrogen). This is a serum-free solution with no animal products added.


COS banker series flyer download [PDF]

Feature and Advantages

  • No dilutions required
  • Store in -80°C freezer
  • Rapid and long term storage at -80°C
  • No serum
  • No animal products
  • Endotoxin-free tested / Bacteria free tested

Kit component

Cos Banker (120 ml)

  • Contains DMSO 10%
  • No serum
  • Store at 2-10 degrees of dark place
  • Expiration date: 2 years from date of manufacture

Applications tested

  • P3UI (mouse myeloma cells)
  • OKT4 (mouse hybridoma cells)
  • PC12 (human neuronal cells)
  • K562 (human leukemia cells)
  • Other human and mouse lymphocytes

Instructions

  1. Collect culture cells (during logarithmic growth stage) and place 5x105 - 1x107 cells in a cryovial. Centrifuge for 5 minutes at 1,200 rpm.
  2. Add 1 ml of Cos Banker to cell pellet and vigorously mix, then freeze and store at -80°C.
  3. If liquid Nitrogen storage is desired, freeze cells at -80°C first and then transfer to liquid Nitrogen.
  4. To thaw, defrost in 37°C water bath quickly.

Note: It is ideal to use a logarithmic growth stage culture in order to increase the survival rate when thawed.

Product List

Product Name Cat# Quantity Price

COS banker [Cell Freezing Medium] (chemical difined) DataSheet ,

KOJ-COS-CFM01

120 ML ¥12,000
$160

COS banker II [Cell Freezing Medium] DataSheet ,

KOJ-COS-CFM02

120 ML ¥12,000
$160

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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