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Collagen solutions for cell and tissue culturing

Atelocollagen : pepsin-solubilized type I collagen solution
Native collagen : acid-solubilized type I collagen solution


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Background

Collagen solution for cell and tissue culturing is highly Purified collagen solution manufactured in KOUKEN (Tokyo) advanced technology with excellent quality control.

Cell and tissue cultures with Koken collagen solutions can express cellular functions like in vivo cells. Collagen not only plays the role of physical scaffold for the proliferating cells, but also enhances cell adhesion, proliferation and function. Collagen creates a natural extracellular environment that is important for cell communication and layer formation. The synthesizing capability of albumin, a functions shared among hepatocytes, is maintained at a high level when cultured on plates coated with collagen solution. When skin fibroblasts are cultured three-dimensionally on collagen gel , the whole collagen contracts from the contractility of the cells producing a dermis model that closely resembles the structure of live dermis.


AteloCell® flyer download [PDF]

Features and Advantages

1. All products are sterilized. The used collagen are extracted from bovine dermis, solubilized and purified by our original methods.
2. Acidic solutions, I-PC and I-AC, form fibrils when being neutralized at 37°C

Application

# Collagen coating of culture plates
# Three dimensional culture in a collagen gel
# Culture on a gel

Instructions for preparing collagen solutionsPDF

References

1) An In vitro Multistep Carcinogenesis Model for Human Cervical Cancer. Narisawa-Saito M, Yoshimatsu Y, Ohno S, et al. Cancer Res. 2008 Jul 15;68(14):5699-705

2) Proteomic analysis of hypoxia-induced tube breakdown of an in vitro capillary model composed of HUVECs : Potential role of p38-regulated reduction of HSP27. Eguchi R, Naitou H, Kunimasa K, et al.Proteomics. 2008 Jul;8(14):2897-906

3) Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities. Hannachi Imen E, Nakamura M, Mie M, Kobatake E. J Biotechnol. 2009 Jan 1;139(1) :19-25

4) Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells. Eguchi R, Tone S, Suzuki A, et al., Exp Cell Res. 2009 Jan 15;

Product List

Product Name Cat# Quantity Price

Atelocollagen, Bovine dermis, 3mg/mL DataSheet ,

KOU-IPC-30

50 ML ¥9,000
$120

Atelocollagen, Bovine dermis, 5mg/mL DataSheet ,

KOU-IPC-50

50 ML ¥13,500
$180

Native collagen, Bovine dermis, 3mg/mL DataSheet ,

KOU-IAC-30

50 ML ¥14,600
$195

Native collagen, Bovine dermis, 5mg/mL DataSheet ,

KOU-IAC-50

50 ML ¥23,700
$316

Atelocollagen powder

Atelocollagen powder for research is a highly purified, bovine dermal type I atelocollagen applicable to various purposes.
* Since the product will be manufactured upon order receipt please inquire about the date of delivery.


Product Name Cat# Quantity Price

Atelocollagen powder, Bovine DataSheet

KOU-CLP-01

500 MG ¥32,400
$432

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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