useful tool for three dimensional culture and research of tissue engineering as 3D scaffold

Atelocollagen, Honeycomb sponge


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Background

 

The 'Honeycomb' collagen sponge has a structure in which uniform pores (200-400 μm) are arranged densely in one direction, into which cells can penetrate and proliferate. This structure facilitates the ready supply of nutrients to the cells inside the sponge, and releases metabolic wastes and biochemical products. Cells can proliferate and fill the lumen to form a uniform cell mass.

Atelocollagen Honeycomb Sponge (KOU-CSH-10) is a 3 mm cube with applications including cell scaffolding for 3-D cell culture and high density cell culture substrate for tissue engineering.

KOU_CSH96_1.jpg

Honeycomb Disk 96 (KOU-CSH-96) is a disk-shaped atelocollagen sponge with a diameter of 6 mm, ideal for high density culture in 96-wel l plates and high throughput screening.


KOU-CSH-10 : stereoscopic microscope image

KOU_CSH96_2.jpg
KOU-CSH-96 : stereoscopic microscope image


Electron microscope image of Honeycomb sponge


Electron microscope image of mouse fibroblast cell culture in 'Honeycomb collagen sponge

 

Characteristics of Atelocollagen FAQ
Atelocollagen is a collagen solubilized by protease, but its physical properties are virtually identical to those of natural, unsolubilized collagen. Furthermore, atelocollagen additionally has superior characteristics;----- More

Characteristics of Atelocollagen FAQ


Honeycomb Sponge flyer download [PDF]

AteloCell® flyer download [PDF]

 

Feature and Advantages

The 'Honeycomb' collagen sponge is prepared from highly purified bovine dermal type I Atelocollagen and can be degraded by collagenase.

Application

Three dimensional culture
Research for tissue engineering as 3D scaffold

Instruction manual for Atelocollagen, Honeycomb sponge PDF

 

♦ Experimental example 1

 

Experimental example 2

Suppression of embroyoid body teratogenesis following in vivo transplantation of EB on a Honeycomb Sponge scaffold.

Mouse kidney at 12 weeks following transplantation of EB on KOU-CSH-10 Honeycomb Sponge scaffold (EB/+CSH) showed no sign of teratoma formation while EB transplanted without KOU-CSH-10 (ES/-CSH) generated teratomas in all mice. Histologically, transplanted ES/+CSH were indistinguishable from adjacent host renal tissue, suggesting spontaneous differentiation without specific induction.
Methods: Plate cultured mouse embryonic stem (ES) cells were trypsinized, filtered through nylon mesh, seeded into 96-well plates (1x104 cells/well), and cultured for 5 days to form embryoid bodies (EB). When EB were mixed with Honeycomb Sponge (KOU-CSH-10), EB rapidly and uniformly integrated into the KOU-CSH-10 matrix.
EB/+CSH complex was then transplanted under renal fascia of 6-week-old mice.

> Research brief

 

Experimental example 3

De novo generation of hair following in vivo transplantation of mouse ES/mesenchymal-cell mosaic spheres on a KOU-CSH-10 scaffold.

Methods: Mouse ES cells and fetal mouse mesenchymal cells (MDU1) were co-cultured to produce two-cell mosaic spheres. The mosaic spheres were mixed with KOU-CSH-10 Atelocollagen Honeycomb Sponge and transplanted under the back muscles of 6-week-old mice.

<References>
1. Suzuki T, et al. Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs. (2009) Microvasc Res. 77(2):143-149.

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Product List

Product Name Cat# Quantity Price

Atelocollagen Honeycomb sponge DataSheet ,

KOU-CSH-10

100 MG ¥27,000
$360

Atelocollagen Honeycomb Disc 96 DataSheet ,

KOU-CSH-96

25 PC ¥28,000
$374

KOU-CSH-10
Size  :  3 × 3 × 2 mm/sponge
Total surface area  :  about 2000 cm²/g
Weight per volume: 12∼13 mg/mL
Pore size : 200 - 400μm Sterile

KOU-CSH-96
Size  :  φ6×2mm
Pore size : 200 - 400μm Sterile


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