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  • STELLA+ "Lysine Labeling Kit" [TAMRA, BIOTIN, DOTA, HiLyte Fluor 647, HiLyte Fluor 750]

Useful for selective labeling the lysine residue or ethanol amine

STELLA+ "Lysine Labeling Kit" [TAMRA, BIOTIN, DOTA, HiLyte Fluor 647, HiLyte Fluor 750]


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Background

STELLA+ Lysine Labeling Kit is a labeling kit to selectively label the lysine residue of protein (antibody) or the lysine residue and ethanol amine localized on cell surfaces.

Unlike the conventional labeling method with use of succinimidyl ester compound, this kit is capable of rapid and efficient labeling at low concentration by a newly developed 6π-Azaelectrocyclization reaction process of hexatriene-β-carbonyl compound.
Moreover, since it reacts only with the lysine residue on the protein surface and does not react with N-end amino group or lysine residue group, which is indispensable for interaction of receptors, it can perform labeling efficiently without spoiling the function of biopolymers.

Caution : Please do not use reagents containing amino groups or lysines while using the Laveling Kit.


STELLA+ -Lysin Labelling Kit Flyer download [PDF]

Principle

<Rapid 6 -Azaelectrocyclization process>

<Spectral Properties>
Fluorescent dye Ex/Em
TAMRA 543 nm/570 nm
HiLyte Fluor 647 649 nm/669 nm
HiLyte Fluor 750 754 nm/778 nm

Feature and Advantages

# This kit is capable of rapid and efficient labeling by 6π-Azaelectrocyclization reaction process of hexatriene-β-carbonyl compound.

# This kit reacts only with the lysine residue on the protein surface and the bond
Is covalent bond.

# Since it does not react with N-end amino group or lysine residue group, which is indispensable for interaction of receptors,the function of biomolecules is not lost.

# In the sample of very low concentration, it can perform labeling efficiently.
When the concentration of the reagent is used by about ten times, the consentration of the slabeling sample can be decreased to 10-8M or less level

Application

<Cell staining>

 

<Scheme>
“In Eppendorf-tube” preparation method of unsaturated aldehyde probes and fluorescence labeling of the cells.

 

<Confocal microscopy of the TAMRA-labeled C6 glioma cells (excitation at 525 nm)>
As a blue dye, DAPI is introduced After fixing the TAMRA-labeled cell by paraformaldehyde treatments.@2D- and 3D-pictures of a labeled cell by (a) unsaturated aldehyde probe 1a and (b) TAMRA-succinimidyl ester at 1 x 10-5 M, 37Ž for 10 min. Labeling perfomed at 1 x 10-8 M, 37Ž for 10 min by (C) 1a and (d) TAMRA-succinimidyl ester.

KSD_99000028_2.JPG
Details of this data will be published in Chemmed Chem. soon.
Tanaka, K. Minami, K. Tahara, T. Fujii, Y., E. R. O. Siwu, Nozaki, S. Onoe, H. Yokoi, S. Koyama, K. Watanabe, Y. and Fukase, K.
Electrocyclization-Based Labeling Allows Efficient In Vivo Imaging of Cellular Trafficking Chemmed Chem. 2010

 

<Fluorescence imaging of lymphocytes in mice>
Labeled and /or engineered cells were administrated intravenously (n=3, 100 ul/mouse, 104 cells) and whole body was scanned from the back side 1h, 2h, 4h, 6h, 48h and 1 week after injection. Data were normalized. SP:spleen; LN:Lymph node of epidermal intestinal tract; TM:DLD-1 human colon carcinoma.

KSD_99000028_3.JPG
Details of this data will be published in J. Carbohydr. Chem. soon.
Tanaka, K. Minami, K. Tahara, T., E. R. O. Siwu, Koyama, K. Nozaki, S. Onoe, H. Watanabe, Y. and Fukase, K.
A Combined 6π-Azaelectrocyclization/Staudinger Approach to Protein and Cell Engineering: Non-Invasive Tumor Targeting by N-Glycan-Engineered Lymphocytes
J. Carbohydr. Chem. 2010

 

< Procedure and content>
Instruction manualPDF

 

<References>
1. Tanaka, K. ; Katsumura, S. J. Synth.Org.Chem. Japan.1999, 55, 1657.
2. Tanaka, K. ; Katsumura, S. J. Am.Chem.Soc.2002, 124, 9660.
3. Tanaka, K. ; Katsumura, S. J. Synth. Org. Chem. Japan. 2005, 63, 696.
4. Tanaka, K. ; Masuyama, T. ; Hasegawa, K. ; Tahara, T. ; Mizuma, H. ; Wada, Y. ; Watanabe, Y. ; Fukase, K. Angew. Chem. Int. Ed. 2008, 47, 102-105.
5. Tanaka, K. ; Fukase, K. Org. Biomol. Chem. 2008, 6, 815-828.

Product List

Special Offer : Trial gSTELLA+ Lysine Labeling kith [ 1kit ]

NOTE : once in one laboratory

Product Name Cat# Quantity Price

STELLA+ Lysine Labeling kit TAMRA Sample DataSheet ,

KSD-990-00028S

1 KIT N/A

STELLA+ Lysine Labeling kit DOTA Sample DataSheet ,

KSD-990-00036S

1 KIT N/A

STELLA+ Lysine Labeling kit HiLyte Fluor 647 Sample DataSheet ,

KSD-990-00044S

1 KIT N/A

STELLA+ Lysine Labeling kit HiLyte Fluor 750 Sample DataSheet ,

KSD-990-00045S

1 KIT N/A

STELLA+ "Lysine Labeling Kit"

Note : Product "KSD-990-00036" cannot be sold to USA because of the patent issue.

Product Name Cat# Quantity Price

STELLA+ Lysine Labeling kit TAMRA DataSheet ,

KSD-990-00028

1 KIT N/A

STELLA+ Lysine Labeling kit BIOTIN DataSheet ,

KSD-990-00035

1 KIT N/A

STELLA+ Lysine Labeling kit DOTA DataSheet ,

KSD-990-00036

1 KIT N/A

STELLA+ Lysine Labeling kit HiLyte Fluor 647 DataSheet ,

KSD-990-00044

1 KIT N/A

STELLA+ Lysine Labeling kit HiLyte Fluor 750 DataSheet ,

KSD-990-00045

1 KIT N/A

“STELLA+ "Lysine Labeling Kit" [TAMRA, BIOTIN, DOTA, HiLyte Fluor 647, HiLyte Fluor 750]” also belongs to the following categories.